Cell Wall Modifications during Conidial Maturation of the Human Pathogenic Fungus Pseudallescheria boydii
| Autor: | Ghamrawi, Sarah, Rénier, Gilles, Saulnier, Patrick, Cuenot, Stéphane, Zykwinska, Agata, Dutilh, Bas E, Thornton, Christopher, Faure, Sébastien, Bouchara, Jean-Philippe, Sub Bioinformatics, Theoretical Biology and Bioinformatics |
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| Přispěvatelé: | Univ Angers, Okina, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), Micro et Nanomédecines Biomimétiques (MINT), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL), LUNAM / Ecole des Mines de Nantes / IRCCyN (EMN), Mines Nantes (Mines Nantes), Universidade Federal do Rio de Janeiro (UFRJ), University of Exeter, Stress Oxydant et Pathologies Métaboliques (SOPAM), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Sub Bioinformatics, Theoretical Biology and Bioinformatics |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Pigments
[SDV]Life Sciences [q-bio] Fungal Physiology lcsh:Medicine Cell Wall/metabolism Virulence Factors/biosynthesis Naphthols Conidium Melanin Pseudallescheria Cell Wall Microbial Physiology Lectins Tumours of the digestive tract Radboud Institute for Molecular Life Sciences [Radboudumc 14] lcsh:Science Fungal Biochemistry Pseudallescheria/metabolism Fungal Pathogens Multidisciplinary biology Melanins/metabolism Fungal genetics Virulence Factors/metabolism Naphthols/pharmacology Pseudallescheria/cytology Pathogenic fungus Spores Fungal Pseudallescheria/drug effects [SDV] Life Sciences [q-bio] Fungal/drug effects” Medical Microbiology Physical Sciences Pseudallescheria/physiology Research Article Surface Properties Virulence Factors Materials Science Static Electricity Melanins/biosynthesis Mycology Microbiology Cell wall Immune system Polysaccharides Culture Techniques Humans Polysaccharides/metabolism Fungal/physiology*” Microbial Pathogens Materials by Attribute Cell Wall/drug effects Melanins Glycoconjugates/metabolism Organic Pigments lcsh:R fungi “Spores Biology and Life Sciences biology.organism_classification Pseudallescheria boydii Lectins/metabolism lcsh:Q Glycoconjugates |
| Zdroj: | PLoS ONE PLoS One, 9(6). Public Library of Science PLoS ONE, 2014, 9 (6), pp.e100290. ⟨10.1371/journal.pone.0100290⟩ PLoS ONE, Public Library of Science, 2014, 9 (6), pp.e100290. ⟨10.1371/journal.pone.0100290⟩ PLoS One, 9 PLoS ONE, Vol 9, Iss 6, p e100290 (2014) PLoS One, 9, 6 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0100290⟩ |
| Popis: | Contains fulltext : 136222.pdf (Publisher’s version ) (Open Access) Progress in extending the life expectancy of cystic fibrosis (CF) patients remains jeopardized by the increasing incidence of fungal respiratory infections. Pseudallescheria boydii (P. boydii), an emerging pathogen of humans, is a filamentous fungus frequently isolated from the respiratory secretions of CF patients. It is commonly believed that infection by this fungus occurs through inhalation of airborne conidia, but the mechanisms allowing the adherence of Pseudallescheria to the host epithelial cells and its escape from the host immune defenses remain largely unknown. Given that the cell wall orchestrates all these processes, we were interested in studying its dynamic changes in conidia as function of the age of cultures. We found that the surface hydrophobicity and electronegative charge of conidia increased with the age of culture. Melanin that can influence the cell surface properties, was extracted from conidia and estimated using UV-visible spectrophotometry. Cells were also directly examined and compared using electron paramagnetic resonance (EPR) that determines the production of free radicals. Consistent with the increased amount of melanin, the EPR signal intensity decreased suggesting polymerization of melanin. These results were confirmed by flow cytometry after studying the effect of melanin polymerization on the surface accessibility of mannose-containing glycoconjugates to fluorescent concanavalin A. In the absence of melanin, conidia showed a marked increase in fluorescence intensity as the age of culture increased. Using atomic force microscopy, we were unable to find rodlet-forming hydrophobins, molecules that can also affect conidial surface properties. In conclusion, the changes in surface properties and biochemical composition of the conidial wall with the age of culture highlight the process of conidial maturation. Mannose-containing glycoconjugates that are involved in immune recognition, are progressively masked by polymerization of melanin, an antioxidant that is commonly thought to allow fungal escape from the host immune defenses. |
| Databáze: | OpenAIRE |
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