Excited-State Dynamics in Borylated Arylisoquinoline Complexes in Solution and in cellulo
| Autor: | Tingxiang Yang, Abha Valavalkar, Antonio Romero‐Arenas, Anindita Dasgupta, Patrick Then, Avinash Chettri, Christian Eggeling, Abel Ros, Uwe Pischel, Benjamin Dietzek‐Ivanšić |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| DOI: | 10.1002/chem.202203468 |
| Popis: | Two four-coordinate organoboron N,C-chelate complexes with different functional terminals on the PEG chains are studied with respect to their photophysical properties within human MCF-7 cells. Their excited-state properties are characterized by time-resolved pump-probe spectroscopy and fluorescence lifetime microscopy. The excited-state relaxation dynamics of the two complexes are similar when studied in DMSO. Aggregation of the complexes with the carboxylate terminal group is observed in water. When studying the light-driven excited-state dynamics of both complexes in cellulo, i. e., after being taken up into human MCF-7 cells, both complexes show different features depending on the nature of the anchoring PEG chains. The lifetime of a characteristic intramolecular charge-transfer state is significantly shorter when studied in cellulo (360±170 ps) as compared to in DMSO (∼960 ps) at 600 nm for the complexes with an amino group. However, the kinetics of the complexes with the carboxylate group are in line with those recorded in DMSO. On the other hand, the lifetimes of the fluorescent state are almost identical for both complexes in cellulo. These findings underline the importance to evaluate the excited-state properties of fluorophores in a complex biological environment in order to fully account for intra- and intermolecular effects governing the light-induced processes in functional dyes. This work was supported by the European Union (via the ITN LogicLab funded under the Horizon 2020 research and innovation program under the grant agreement No 813920). We thank Prof. Dr. Rainer Heintzmann and Dr. Benedict Diederich for providing BioLab facilities and supporting the image acquisition. The ELYRA 7 (used for producing Figure 3 and S8) was funded by the Free State of Thuringia with grant number 2019 FGI 0003 and supported by the Microverse Imaging Center (funded by the DFG under Germany Âs Excellence Strategy �R EXC 2051 �R Project-ID 390713860). We further acknowledge funding by the DFG (Project number 316213987 – SFB 1278, INST 1757/25-1 FUGG), the Free State of Thuringia (TAB, TMWWDG, AdvancedSTED/2018 FGI 0022; Advanced Flu-Spec/ 2020 FGI 0031), BMBF (Photonics Research Germany (FKZ; 3N15713/13N15717) integrated into the Leibniz Center for Photonics in Infection Research (LPI)) and the innovation program by the German BMWi (ZIM; project 16KN070934 / Labon- a-chip FCS-Easy). The Spanish Ministerio de Ciencia e Innovación (grants PID2020-119992GB-I00, PID2019-106358GBC21, and PID2019-106358GB-C22), the Consejo Superior de Investigaciones Científicas (grant 202080I005 for A.R.), and the Junta de Andalucía/University of Huelva (grant UHU-202070) are thanked for financial support. We thank Dr. Z. Dom Nguez for assistance in the early stage of this project. Open Access funding enabled and organized by Projekt DEAL. |
| Databáze: | OpenAIRE |
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