The MBD7 complex promotes expression of methylated transgenes without significantly altering their methylation status
| Autor: | Julie A. Law, Brandon H. Le, Dongming Li, Lei Gao, Ying Feng, Shengben Li, Jinyuan Liu, Beixin Mo, Li Liu, Xigang Liu, Ana Marie S. Palanca, Xiuyun Wu, Ajay A. Vashisht, So Youn Won, Guodong Yang, James A. Wohlschlegel, Yun Ju Kim, Shaofang Li, Xuemei Chen, Hongwei Guo, Yuanyuan Zhao |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
α-crystallin domain Arabidopsis Plant Biology Gene Expression Regulation Plant Genes Reporter Histone methylation Transgenes Biology (General) Luciferases RNA-Directed DNA Methylation Epigenomics Plant Proteins plant biology Genetics Regulation of gene expression DNA methylation General Neuroscience General Medicine DNA-Binding Proteins HSP20 Genes and Chromosomes Medicine transcriptional gene silencing Research Article chromosomes QH301-705.5 Science Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Epigenetics of physical exercise α-crystallin domain (ACD) Methyl-CpG-Binding Domain (MBD) Epigenetics Reporter Post-transcriptional regulation Methyl-CpG-Binding Domain General Immunology and Microbiology Human Genome RNA-directed DNA methylation RNA-directed DNA methylation (RdDM) Plant 030104 developmental biology Gene Expression Regulation Genes A. thaliana Generic health relevance Biochemistry and Cell Biology |
| Zdroj: | eLife eLife, Vol 6 (2017) |
| ISSN: | 2050-084X |
| Popis: | DNA methylation is associated with gene silencing in eukaryotic organisms. Although pathways controlling the establishment, maintenance and removal of DNA methylation are known, relatively little is understood about how DNA methylation influences gene expression. Here we identified a METHYL-CpG-BINDING DOMAIN 7 (MBD7) complex in Arabidopsis thaliana that suppresses the transcriptional silencing of two LUCIFERASE (LUC) reporters via a mechanism that is largely downstream of DNA methylation. Although mutations in components of the MBD7 complex resulted in modest increases in DNA methylation concomitant with decreased LUC expression, we found that these hyper-methylation and gene expression phenotypes can be genetically uncoupled. This finding, along with genome-wide profiling experiments showing minimal changes in DNA methylation upon disruption of the MBD7 complex, places the MBD7 complex amongst a small number of factors acting downstream of DNA methylation. This complex, however, is unique as it functions to suppress, rather than enforce, DNA methylation-mediated gene silencing. DOI: http://dx.doi.org/10.7554/eLife.19893.001 |
| Databáze: | OpenAIRE |
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