Xanthomonas prunicola sp. nov.;a novel pathogen that affects nectarine (Prunus persica var. nectarina) trees
| Autor: | Ester Marco-Noales, Marion Fischer-Le Saux, Carmen Beltrán, Pablo López-Soriano, Jaime Cubero, Perrine Portier, María M. López, Jerson Garita-Cambronero, Géraldine Taghouti |
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| Přispěvatelé: | Instituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research (IVIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ministerio de Cienca e Innovacion, Instituto Tecnológico Agrario de Castilla y León, Consejeria de Agricultura y Pesca, IFAPA-Centro El Toruño, Institut de Recherche en Horticulture et Semences (IRHS), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA) RTA2011-00140-C03-00, INIA (FPI-INIA), Valencian Institute of Agrarian Research (IVIA), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA) |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine DNA Bacterial MLSA Xanthomonas Sequence analysis phenotypic characterization 01 natural sciences Microbiology Trees 03 medical and health sciences Prunus Genus RNA Ribosomal 16S Botany Pathogen Ecology Evolution Behavior and Systematics Phylogeny Plant Diseases Prunus persica new species biology Pigmentation Strain (biology) Fatty Acids General Medicine Xanthomonas arboricola Sequence Analysis DNA 16S ribosomal RNA biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Bacterial Typing Techniques Plant Leaves 030104 developmental biology Spain Fruit polyphasic approach ANI 010606 plant biology & botany Multilocus Sequence Typing |
| Zdroj: | Repositorio de Resultados de Investigación del INIA INIA: Repositorio de Resultados de Investigación del INIA International Journal of Systematic and Evolutionary Microbiology International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2018, 68 (6), pp.68:1857-1866. ⟨10.1099/ijsem.0.002743⟩ International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2018, pp.68:1857-1866. ⟨10.1099/ijsem.0.002743 ⟩ International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2018, pp.68:1857-1866. ⟨10.1099/ijsem.0.002743⟩ |
| ISSN: | 1466-5026 1466-5034 |
| DOI: | 10.1099/ijsem.0.002743⟩ |
| Popis: | International audience; Three isolates obtained from symptomatic nectarine trees (Prunus persica var. nectarina) cultivated in Murcia, Spain, which showed yellow and mucoid colonies similar to Xanthomonas arboricola pv. pruni, were negative after serological and real-time PCR analyses for this pathogen. For that reason, these isolates were characterized following a polyphasic approach that included both phenotypic and genomic methods. By sequence analysis of the 16S rRNA gene, these novel strains were identified as members of the genus Xanthomonas, and by multilocus sequence analysis (MLSA) they were clustered together in a distinct group that showed similarity values below 95 % with the rest of the species of this genus. Whole-genome comparisons of the average nucleotide identity (ANI) of genomes of the strains showed less than 91 % average nucleotide identity with all other species of the genus Xanthomonas. Additionally, phenotypic characterization based on API 20 NE, API 50 CH and BIOLOG tests differentiated the strains from the species of the genus Xanthomonas described previously. Moreover, the three strains were confirmed to be pathogenic on peach (Prunus persica), causing necrotic lesions on leaves. On the basis of these results, the novel strains represent a novel species of the genus Xanthomonas, for which the name Xanthomonas prunicola is proposed. The type strain is CFBP 8353 (=CECT 9404=IVIA 3287.1). |
| Databáze: | OpenAIRE |
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