Drk-mediated signaling to Rho kinase is required for anesthesia-resistant memory in Drosophila
| Autor: | Vasileia Kotoula, Efthimios M. C. Skoulakis, Ourania Semelidou, Anastasios Moressis |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell signaling Drug Resistance Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine Memory Animals Drosophila Proteins Cytoskeleton Rho-associated protein kinase Mushroom Bodies Anesthetics Anesthesia-resistant memory rho-Associated Kinases Multidisciplinary biology Signal transducing adaptor protein Biological Sciences Actins Cell biology Drosophila melanogaster 030104 developmental biology Mushroom bodies biology.protein GRB2 Signal transduction 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences. 114:10984-10989 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1704835114 |
| Popis: | Anesthesia-resistant memory (ARM) was described decades ago, but the mechanisms that underlie this protein synthesis-independent form of consolidated memory in Drosophila remain poorly understood. Whether the several signaling molecules, receptors, and synaptic proteins currently implicated in ARM operate in one or more pathways and how they function in the process remain unclear. We present evidence that Drk, the Drosophila ortholog of the adaptor protein Grb2, is essential for ARM within adult mushroom body neurons. Significantly, Drk signals engage the Rho kinase Drok, implicating dynamic cytoskeletal changes in ARM, and this is supported by reduced F-actin in the mutants and after pharmacological inhibition of Drok. Interestingly, Drk-Drok signaling appears independent of the function of Radish (Rsh), a protein long implicated in ARM, suggesting that the process involves at least two distinct molecular pathways. Based on these results, we propose that signaling pathways involved in structural plasticity likely underlie this form of translation-independent memory. |
| Databáze: | OpenAIRE |
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