Detection of pancreatic carcinomas by imaging lactose-binding protein expression in peritumoral pancreas using [18F]fluoroethyl-deoxylactose PET/CT
| Autor: | Hsin Hsin Yeh, Uday Mukhopadhyay, Suren Soghomonyan, Craig D. Logsdon, Ashutosh Pal, Yunming Ying, Susanna Bertolini, Andrei Volgin, Mian M. Alauddin, Aleksandr Shavrin, William G. Bornmann, Daniel Young, William P. Tong, Juri G. Gelovani, Leo G. Flores |
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| Rok vydání: | 2009 |
| Předmět: |
Pathology
Fluorine Radioisotopes Gastroenterology and Hepatology/Pancreas lcsh:Medicine Lactose Pancreatitis-Associated Proteins Ligands Mice 0302 clinical medicine lcsh:Science 0303 health sciences Multidisciplinary medicine.diagnostic_test 3. Good health medicine.anatomical_structure Positron emission tomography 030220 oncology & carcinogenesis Pancreas Preclinical imaging Research Article medicine.medical_specialty Gastroenterology and Hepatology/Gastrointestinal Cancers Oncology/Gastrointestinal Cancers Models Biological 03 medical and health sciences Antigens Neoplasm Pancreatic cancer medicine Carcinoma Biomarkers Tumor Animals Lectins C-Type 030304 developmental biology Tomography Emission-Computed Single-Photon PET-CT business.industry Carcinoma Acinar Cell lcsh:R Lactose binding medicine.disease Pancreatic Neoplasms Positron-Emission Tomography Radiology and Medical Imaging/PET and SPECT Imaging lcsh:Q Radiopharmaceuticals business Tomography X-Ray Computed Neoplasm Transplantation |
| Zdroj: | PLoS ONE PLoS ONE, Vol 4, Iss 11, p e7977 (2009) |
| ISSN: | 1932-6203 |
| Popis: | Background Early diagnosis of pancreatic carcinoma with highly sensitive diagnostic imaging methods could save lives of many thousands of patients, because early detection increases resectability and survival rates. Current non-invasive diagnostic imaging techniques have inadequate resolution and sensitivity for detection of small size (∼2–3 mm) early pancreatic carcinoma lesions. Therefore, we have assessed the efficacy of positron emission tomography and computer tomography (PET/CT) imaging with β-O-D-galactopyranosyl-(1,4′)-2′-deoxy-2′-[18F]fluoroethyl-D-glucopyranose ([18F]FEDL) for detection of less than 3 mm orthotopic xenografts of L3.6pl pancreatic carcinomas in mice. [18F]FEDL is a novel radioligand of hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein (HIP/PAP), which is overexpressed in peritumoral pancreatic acinar cells. Methodology/Principal Findings Dynamic PET/CT imaging demonstrated rapid accumulation of [18F]FEDL in peritumoral pancreatic tissue (4.04±2.06%ID/g), bi-exponential blood clearance with half-lives of 1.65±0.50 min and 14.14±3.60 min, and rapid elimination from other organs and tissues, predominantly by renal clearance. Using model-independent graphical analysis of dynamic PET data, the average distribution volume ratio (DVR) for [18F]FEDL in peritumoral pancreatic tissue was estimated as 3.57±0.60 and 0.94±0.72 in sham-operated control pancreas. Comparative analysis of quantitative autoradiographic images and densitometry of immunohistochemically stained and co-registered adjacent tissue sections demonstrated a strong linear correlation between the magnitude of [18F]FEDL binding and HIP/PAP expression in corresponding regions (r = 0.88). The in situ analysis demonstrated that at least a 2–4 fold apparent lesion size amplification was achieved for submillimeter tumors and to nearly half a murine pancreas for tumors larger than 3 mm. Conclusion/Significance We have demonstrated the feasibility of detection of early pancreatic tumors by non-invasive imaging with [18F]FEDL PET/CT of tumor biomarker HIP/PAP over-expressed in peritumoral pancreatic tissue. Non-invasive non-invasive detection of early pancreatic carcinomas with [18F]FEDL PET/CT imaging should aid the guidance of biopsies and additional imaging procedures, facilitate the resectability and improve the overall prognosis. |
| Databáze: | OpenAIRE |
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