Magnesium deficiency regulates vitamin D metabolizing enzymes and type II sodium-phosphate cotransporter mRNA expression in rats
| Autor: | Hiroshi Matsuzaki, Misao Miwa, Yasutaka Kajita, Shin-ichi Katsumata |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty animal structures Clinical Biochemistry chemistry.chemical_element Sodium-Phosphate Cotransporter Proteins Type IIc Calcium Biochemistry Sodium-Phosphate Cotransporter Proteins Type IIb Downregulation and upregulation Internal medicine medicine Pi Vitamin D and neurology Animals RNA Messenger Rats Wistar Vitamin D3 24-Hydroxylase Molecular Biology Kidney Magnesium Metabolism Rats Endocrinology medicine.anatomical_structure chemistry Gene Expression Regulation Steroid Hydroxylases Cotransporter Magnesium Deficiency |
| Zdroj: | Magnesium research. 26(2) |
| ISSN: | 1952-4021 |
| Popis: | A magnesium (Mg) deficiency induces changes in calcium (Ca) and phosphorus (P) metabolism; however, the mechanisms responsible for these effects remain unclear. Since 1,25-dihydroxyvitamin D3 and type II sodium-phosphate (Na/Pi) cotransporters are essential regulators of Ca and P metabolism, this study examined the effects of Mg deficiency on the mRNA expression of vitamin D metabolizing enzymes (25-hydroxyvitamin D-1α-hydroxylase (1α(OH)ase) and 25-hydroxyvitamin D-24-hydroxylase (24(OH)ase)), and Na/Pi cotransporters (type IIa and IIc) in the rat kidney. Rats were divided into two groups and fed a control diet (Mg concentration: 0.05%) or a Mg-deficient diet (Mg concentration: Mg-free) for 21 days. 1α(OH)ase mRNA levels were significantly decreased in rats fed the Mg-deficient diet, while 24(OH)ase mRNA levels were significantly increased, compared to rats fed the control diet. Type IIa and IIc Na/Pi cotransporter mRNA levels in rats fed the Mg-deficient diet were significantly decreased compared to rats fed the control diet. These results suggest that Mg deficiency induces downregulation of 1α(OH)ase and type IIa and IIc Na/Pi cotransporters, and upregulation of 24(OH)ase in the kidney. |
| Databáze: | OpenAIRE |
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