MIP-1α and TGF-β Production in CD34+ Progenitor–Stromal Cell Coculture Systems: Effects of Progenitor Isolation Method and Cell–Cell Contact
| Autor: | Abigail W. Harbol, Todd Belanger, Camille N. Abboud, Jane L. Liesveld, Karen Rosell |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Stromal cell
medicine.medical_treatment CD34 Antigens CD34 Bone Marrow Cells Cell Communication Cell Separation Biology Transforming Growth Factor beta Tumor Cells Cultured medicine Animals Humans Progenitor cell Chemokine CCL4 Antigen-presenting cell Molecular Biology Macrophage inflammatory protein Cells Cultured Chemokine CCL3 Leukemia Stem Cells Mesenchymal stem cell Cell Biology Hematology Macrophage Inflammatory Proteins Molecular biology Coculture Techniques Cytokine Gene Expression Regulation Immunology RNA Molecular Medicine Calcium Stromal Cells Stem cell |
| Zdroj: | Blood Cells, Molecules, and Diseases. 26:261-275 |
| ISSN: | 1079-9796 |
| DOI: | 10.1006/bcmd.2000.0305 |
| Popis: | Macrophage inflammatory protein-1alpha (MIP-1alpha) is a C-C chemokine which has antiproliferative effects on early hematopoietic progenitors and stimulatory effects on later progenitors. It also possesses chemotactic and activating properties for monocytes, macrophages, and T-cells. CD34+ progenitors isolated utilizing an avidin-biotin immunoadsorption column produced significant amounts of MIP-1alpha from 24 h onward when cultured in medium with 10% fetal calf serum (>200 pg/ml). Such production persisted through 96 h of culture and was greater when such progenitors were cocultured with a preformed marrow stromal layer (4000 pg/ml at 24 h). The production of MIP-1alpha declined over time of coculture with stromal layers, and stromal layers themselves produced minimal MIP-1alpha as detected by ELISA |
| Databáze: | OpenAIRE |
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