Structural flexibility of a DNA hairpin located in the long terminal repeat of the Drosophila 1731 retrotransposon
| Autor: | M. Best-Belpomme, E. Brossalina, A. Slama-Schwok, Valentin V. Vlassov, Y. Demchenko |
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| Rok vydání: | 1998 |
| Předmět: |
Retroelements
Retrotransposon Biology chemistry.chemical_compound Transcription (biology) Complementary DNA Genetics Animals A-DNA Fluorescent Dyes Base Sequence Terminal Repeat Sequences Palindrome DNA Hydrogen-Ion Concentration Long terminal repeat DNA binding site Biochemistry chemistry Biophysics Acridines Nucleic Acid Conformation Thermodynamics Drosophila Oligonucleotide Probes Research Article |
| Zdroj: | Nucleic Acids Research. 26:5142-5151 |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | The structure of the DNA binding site of the Nuclear single-stranded Binding Factor (NssBF), located in the long terminal repeat of the Drosophila 1731 retrotransposon, was investigated by melting temperature experiments, chemical probing and fluorescence measurements using a macrocyclic bis-acridine. The most probable structure of this element, named Bc, mainly involves two hairpins in equilibrium at pH 6.0 at low concentration. The hairpins differ in their apical loop size; 4 and 8 nt. The structural flexibility of Bc probably derives from the three consecutive CATA repeats complementary to the GTAT nucleotides of the palindrome. In contrast, the Bc complementary strand adopts a single hairpin. Since Bc is implicated in repression of transcription via binding of two specific factors, its structural flexibility could be associated with this process. |
| Databáze: | OpenAIRE |
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