A Therapeutic SIV DNA Vaccine Elicits T-Cell Immune Responses, but No Sustained Control of Viremia in SIVmac239-Infected Rhesus Macaques
| Autor: | Diane Hebblewaite, Peter Silvera, Jan zur Megede, Dawn Golightly, John J. Donnelly, Brigitte E Sanders-Beer, Jeffrey B. Ulmer, Ranjana Srivastava, Susan W. Barnett, Abigail Bowlsbey, Dietmar Rabussay, Gillis R. Otten, Lei Zhang, Lourdes Nieves-Duran, Deborah Sites |
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| Rok vydání: | 2008 |
| Předmět: |
Interleukin 2
Cellular immunity Electrochemotherapy T-Lymphocytes T cell Immunology Simian Acquired Immunodeficiency Syndrome Viremia Biology medicine.disease_cause Drug Administration Schedule DNA vaccination Virology Vaccines DNA medicine Animals Immunologic Factors Vaccines SAIDS Vaccines Viral Load Simian immunodeficiency virus medicine.disease Combined Modality Therapy Macaca mulatta CD4 Lymphocyte Count Infectious Diseases medicine.anatomical_structure Anti-Retroviral Agents Interleukin-2 Simian Immunodeficiency Virus Viral load CD8 medicine.drug |
| Zdroj: | AIDS Research and Human Retroviruses. 24:1103-1116 |
| ISSN: | 1931-8405 0889-2229 |
| Popis: | The immunologic and virologic outcome of therapeutic DNA-vaccines administered during antiretroviral therapy (ART) using electroporation with or without (interleukin) IL-2 treatment was evaluated in the SIV-mac239/macaque model. Rhesus macaques inoculated with pathogenic SIVmac239 were treated with ART [(R(-9-(2-phosphonomethoxypropyl) adenine) (PMPA), FTC, Zerit®] from weeks 13 to 41 postinfection (wpi). Group 1 (n = 7) received ART only, groups 2 and 3 (each n = 6) additionally received SIVmac239-derived gp140Env, GagPol, and TatRevNef plasmids by in vivo electroporation at 22, 26, 30, and 34 wpi, and group 3 also IL-2 for 14 days after each vaccination. Endpoints evaluated were viral load, Gag181–189-specific CD8+ T-cell responses in MamuA01+ animals, lymphoproliferative responses, and CD4 T-cell counts. Viremia in all animals dropped below 200 RNA copies/ml during ART. Frequencies of Gag181–189-specific CD8+ T cells prior to ART were detectable in all three groups (1.27–3.01%) and increased significantly (p |
| Databáze: | OpenAIRE |
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