POTENTIAL OF BLACK FRUIT SKIN AS ANTIBACTERIAL SOURCE FROM WONDAMA WEST PAPUA

Autor: Yori Turu Toja, Ungirwalu, Antoni, Lestari, Agnes Dyah Novitasari
Jazyk: angličtina
Rok vydání: 2022
Předmět:
DOI: 10.5281/zenodo.7493648
Popis: Black fruit (Haplolobus sp.) is a local fruit that has been part of the Wondama people's tradition from Papua, Indonesia. This fruit is used as sago preservative from generation to generation. In order to increase the usability of black fruit, scientific exploration of this fruit's potential is necessary through proximate analysis, mineral composition, phytochemical analysis, and antibacterial activity testing of the part of this fruit, which in this study was the fruit peel. Proximate and mineral content was analyzed by AOAC methods, the extraction was carried out by maceration, and phytochemical screening was carried out by several testing using phytochemical screening reagents. Whereas the antibacterial assay was carried out by agar disc diffusion assay. The results show that black fruit peel contains high levels of nutrients in dry bases, namely carbohydrates (50.54±0.06%), fat (30.91±0.05%), protein (8.45±0.03%), vitamin C (238.37±1.52 mg/100 g), crude fiber 11.43±0.03%, and ash 4.23±0.01. Minerals content in black fruit from the largest to the smallest levels of K (120.74±2.34 mg/100 g), Ca (19.14±0.95 mg/100 g), Mg (15.47±1.13 mg/100 g), Na (13.13± 0.30 mg/100 g), Fe (3.33±0.31 mg/100 g), Zn (1.61±0.05 mg/100 g), and Cu (1.47±0.03 mg/100 g). Phytochemical analysis reveals that black fruit peel extract contains alkaloids, flavonoids, tannins, polyphenols, triterpenoids, and steroids. The antibacterial activity of the extracts of black fruit peel is more active against Staphylococcus aureus than Escherichia coli. The maximum inhibition zone (9.05 mm) is found at methanol extract at a concentration of 30% toward S. aureus. protein (8.45±0.03%), vitamin C (238.37±1.52 mg/100 g), crude fiber 11.43±0.03%, and ash 4.23±0.01. Minerals content in black fruit from the largest to the smallest levels of K (120.74±2.34 mg/100 g), Ca (19.14±0.95 mg/100 g), Mg (15.47±1.13 mg/100 g), Na (13.13± 0.30 mg/100 g), Fe (3.33±0.31 mg/100 g), Zn (1.61±0.05 mg/100 g), and Cu (1.47±0.03 mg/100 g). Phytochemical analysis reveals that black fruit peel extract contains alkaloids, flavonoids, tannins, polyphenols, triterpenoids, and steroids. The antibacterial activity of the extracts of black fruit peel is more active against Staphylococcus aureus than Escherichia coli. The maximum inhibition zone (9.05 mm) is found at methanol extract at a concentration of 30% toward S. aureus. protein (8.45±0.03%), vitamin C (238.37±1.52 mg/100 g), crude fiber 11.43±0.03%, and ash 4.23±0.01. Minerals content in black fruit from the largest to the smallest levels of K (120.74±2.34 mg/100 g), Ca (19.14±0.95 mg/100 g), Mg (15.47±1.13 mg/100 g), Na (13.13± 0.30 mg/100 g), Fe (3.33±0.31 mg/100 g), Zn (1.61±0.05 mg/100 g), and Cu (1.47±0.03 mg/100 g). Phytochemical analysis reveals that black fruit peel extract contains alkaloids, flavonoids, tannins, polyphenols, triterpenoids, and steroids. The antibacterial activity of the extracts of black fruit peel is more active against Staphylococcus aureus than Escherichia coli. The maximum inhibition zone (9.05 mm) is found at methanol extract at a concentration of 30% toward S. aureus. Minerals content in black fruit from the largest to the smallest levels of K (120.74±2.34 mg/100 g), Ca (19.14±0.95 mg/100 g), Mg (15.47±1.13 mg/100 g), Na (13.13± 0.30 mg/100 g), Fe (3.33±0.31 mg/100 g), Zn (1.61±0.05 mg/100 g), and Cu (1.47±0.03 mg/100 g). Phytochemical analysis reveals that black fruit peel extract contains alkaloids, flavonoids, tannins, polyphenols, triterpenoids, and steroids. The antibacterial activity of the extracts of black fruit peel is more active against Staphylococcus aureus than Escherichia coli. The maximum inhibition zone (9.05 mm) is found at methanol extract at a concentration of 30% toward S. aureus. Minerals content in black fruit from the largest to the smallest levels of K (120.74±2.34 mg/100 g), Ca (19.14±0.95 mg/100 g), Mg (15.47±1.13 mg/100 g), Na (13.13± 0.30 mg/100 g), Fe (3.33±0.31 mg/100 g), Zn (1.61±0.05 mg/100 g), and Cu (1.47±0.03 mg/100 g). Phytochemical analysis reveals that black fruit peel extract contains alkaloids, flavonoids, tannins, polyphenols, triterpenoids, and steroids. The antibacterial activity of the extracts of black fruit peel is more active against Staphylococcus aureus than Escherichia coli. The maximum inhibition zone (9.05 mm) is found at methanol extract at a concentration of 30% toward S. aureus. 47±0.03 mg/100 g). Phytochemical analysis reveals that black fruit peel extract contains alkaloids, flavonoids, tannins, polyphenols, triterpenoids, and steroids. The antibacterial activity of the extracts of black fruit peel is more active against Staphylococcus aureus than Escherichia coli. The maximum inhibition zone (9.05 mm) is found at methanol extract at a concentration of 30% toward S. aureus. 47±0.03 mg/100 g). Phytochemical analysis reveals that black fruit peel extract contains alkaloids, flavonoids, tannins, polyphenols, triterpenoids, and steroids. The antibacterial activity of the extracts of black fruit peel is more active against Staphylococcus aureus than Escherichia coli. The maximum inhibition zone (9.05 mm) is found at methanol extract at a concentration of 30% toward S. aureus.
Databáze: OpenAIRE