Expression profile analysis of 5-day-old neonatal piglets infected with porcine Deltacoronavirus

Autor: Xiao Y. Tang, Kai J. Mai, Zi X. Wu, Qian N. Li, Yuan Sun, Xiang B. Zhang, R.T. Wu, Jing Y. Ma, Di Li, Tian Lan, Jiao L. Wu
Jazyk: angličtina
Rok vydání: 2019
Předmět:
040301 veterinary sciences
Swine
Peroxisome proliferator-activated receptor
Biology
Real-Time Polymerase Chain Reaction
0403 veterinary science
03 medical and health sciences
chemistry.chemical_compound
Intestine
Small

mRNA expression profile
Animals
Gene
PPAR signaling pathway
030304 developmental biology
Oligonucleotide Array Sequence Analysis
chemistry.chemical_classification
Swine Diseases
0303 health sciences
lcsh:Veterinary medicine
Fatty acid metabolism
General Veterinary
Microarray analysis techniques
Fatty acid
Porcine deltacoronavirus
04 agricultural and veterinary sciences
PPAR Pathway
General Medicine
Peroxisome
Cell biology
Coronavirus
Jejunum
Metabolism
chemistry
Animals
Newborn

lcsh:SF600-1100
Signal transduction
Coronavirus Infections
Transcriptome
Metabolic Networks and Pathways
Research Article
Zdroj: BMC Veterinary Research, Vol 15, Iss 1, Pp 1-10 (2019)
BMC Veterinary Research
ISSN: 1746-6148
Popis: Background Porcine deltacoronavirus (PDCoV) is a novel coronavirus that can cause diarrhea in nursing piglets. This study was aimed to investigate the roles of host differentially expressed genes on metabolic pathways in PDCoV infections. Results Twenty thousand six hundred seventy-four differentially expressed mRNAs were identified in 5-day-old piglets responded to PDCoV experimental infections. Many of these genes were correlated to the basic metabolism, such as the peroxisome proliferator-activated receptor (PPAR) signaling pathway which plays a critical role in digestion. At the same time, in the PPAR pathway genes of fatty acid-binding protein (FABP) family members were observed with remarkably differential expressions. The differential expressed genes were associated with appetite decrease and weight loss of PDCoV- affected piglets. Discussion Fatty acid-binding protein 1 (FABP1) and fatty acid-binding protein 3 (FABP3) were found to be regulated by PDCoV. These two genes not only mediate fatty acid transportation to different cell organelles such as mitochondria, peroxisome, endoplasmic reticulum and nucleus, but also modulate fatty acid metabolism and storage as a signaling molecule outside the cell. Therefore, it can be preliminarily concluded that PPAR differential expression caused by PDCoV was mostly associated with weight loss and death from emaciation. Conclusions The host differentially expressed genes were associated with infection response, metabolism signaling and organismal systems signaling pathways. The genes of FABP family members in the PPAR signaling pathway were the most highly altered and played important roles in metabolism. Alteration of these genes were most likely the reason of weight loss and other clinical symptoms. Our results provided new insights into the metabolic mechanisms and pathogenesis of PDCoV infection. Methods Animal experiment, Determination of viral growth by real-time RT-PCR, Histopathology, Immunohistochemical staining, Microarray analysis.
Databáze: OpenAIRE
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