Independent regulation of Plasmodium falciparum rif gene promoters
| Autor: | Diego Peres Alonso, Charlotte Sophie Kaiser, Tatiane Macedo Silva, Gabriela Fernandes Leite, Paulo Eduardo Martins Ribolla, Rosana Beatriz Duque Araujo, Gerhard Wunderlich |
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| Přispěvatelé: | Universidade de São Paulo (USP), Westfalische Wilhelms Univ, Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Plasmodium falciparum Protozoan Proteins lcsh:Medicine Antigens Protozoan Article 03 medical and health sciences Plasmid Transcription (biology) Gene expression parasitic diseases polycyclic compounds Gene family heterocyclic compounds FÁRMACOS lcsh:Science Promoter Regions Genetic Gene Genetics Multidisciplinary biology lcsh:R Promoter biochemical phenomena metabolism and nutrition biology.organism_classification bacterial infections and mycoses Allelic exclusion 030104 developmental biology bacteria lcsh:Q Genome Protozoan |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scientific Reports Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018) |
| Popis: | Made available in DSpace on 2018-11-26T17:52:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-19 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) All Plasmodium species express variant antigens which may mediate immune escape in the vertebrate host. In Plasmodium falciparum, the rif gene family encodes variant antigens which are partly exposed on the infected red blood cell surface and may function as virulence factors. Not all rif genes are expressed at the same time and it is unclear what controls rif gene expression. In this work, we addressed global rif transcription using plasmid vectors with two drug resistance markers, one controlled by a rif 5' upstream region and the second by a constitutively active promoter. After spontaneous integration into the genome of one construct, we observed that the resistance marker controlled by the rif 5' upstream region was expressed dependent on the applied drug pressure. Then, the global transcription of rif genes in these transfectants was compared in the presence or absence of drugs. The relative transcript quantities of all rif loci did not change profoundly between strains grown with or without drug. We conclude that either there is no crosstalk between rif loci or that the elusive system of allelic exclusion of rif gene transcription is not controlled by their 5' upstream region alone. Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Av Prof Lineu Prestes 1374, BR-05508000 Sao Paulo, SP, Brazil Westfalische Wilhelms Univ, Inst Anim Physiol, Schlosspl 8, Munster, Germany State Univ Sao Paulo, IBB IBTEC, Dept Parasitol, Botucatu, SP, Brazil State Univ Sao Paulo, IBB IBTEC, Dept Parasitol, Botucatu, SP, Brazil FAPESP: 2012/23306-5 FAPESP: 2015/17174-7 |
| Databáze: | OpenAIRE |
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