Differential Short-Term Transduction Efficiency of Adult versus Newborn Mouse Tissues by Adenoviral Recombinants
| Autor: | Johnny Huard, Hanns Lochmüller, Agnes Jani, Bernard Massie, Gyula Acsadi, Claude Guérin, George Karpati, Paul C. Holland |
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| Rok vydání: | 1995 |
| Předmět: |
Ratón
Genetic enhancement Genetic Vectors Molecular Sequence Data Clinical Biochemistry Biology medicine.disease_cause Adenoviridae Pathology and Forensic Medicine Mice Transduction (genetics) Antigens CD Genes Reporter medicine Animals pharmaceutical Luciferase Luciferases Molecular Biology Brain Chemistry Electrophoresis Agar Gel Reporter gene Base Sequence Muscles Gene Transfer Techniques Skeletal muscle Integrin alphaV beta-Galactosidase Molecular biology medicine.anatomical_structure Animals Newborn DNA Viral Immunohistochemistry Electrophoresis Polyacrylamide Gel |
| Zdroj: | Experimental and Molecular Pathology. 62:131-143 |
| ISSN: | 0014-4800 |
| DOI: | 10.1006/exmp.1995.1015 |
| Popis: | We demonstrated different transduction efficiency in several major organs of the immature (newborn) versus mature (adult) mice using adenoviral recombinants containing expression cassettes for either firefly luciferase or bacterial β-galactosidase reporter genes. The studied tissues included skeletal muscle, heart, brain, lung, kidney, and liver. The transduction efficiency in all tissues, especially skeletal muscle, was significantly less in adults than in newborns, with two exceptions. In the heart, transduction efficiency was the same in newborns and adults, while in brain, it was greater in the adult than in the newborn. The cited differences in transduction efficiencies between newborn and adult tissues applied approximately equally to both reporter genes. The α v integrin level showed the same trend as the transduction efficiency in all tissues, except the heart. Polymerase chain reaction showed a specific adenoviral product in proportion to the reporter gene expression in muscle, heart, and brain. The results of this study should be considered in designing gene therapy strategies in genetic diseases. |
| Databáze: | OpenAIRE |
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