Colistin stimulates the activity of neutrophil elastase and Pseudomonas aeruginosa elastase
| Autor: | Heather Elphick, G. S. Evans, Pettitt E, Mark L. Everard, A. Jones |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Proteases Serine Proteinase Inhibitors Adolescent Cystic Fibrosis medicine.drug_class Antibiotics In Vitro Techniques medicine.disease_cause Ceftazidime Cystic fibrosis Amino Acid Chloromethyl Ketones Microbiology Bacterial Proteins Tobramycin Humans Medicine Child Respiratory Tract Infections biology Colistin Heparin business.industry Pseudomonas aeruginosa Elastase Sputum Anticoagulants Metalloendopeptidases medicine.disease Anti-Bacterial Agents Erythromycin Enzyme Activation alpha 1-Antitrypsin Neutrophil elastase biology.protein Female Gentamicins Leukocyte Elastase business medicine.drug |
| Zdroj: | European Respiratory Journal. 19:1136-1141 |
| ISSN: | 1399-3003 0903-1936 |
| DOI: | 10.1183/09031936.02.00230602 |
| Popis: | Nonantimicrobial effects of antibiotics may contribute to their activity in the treatment of infective airway disease. The aim of this study was to identify antibiotics used for the treatment of infection in cystic fibrosis that may alter the activity of human neutrophil elastase (HNE) and Pseudomonas aeruginosa elastase (PE). The effect of antibiotics on the activity of purified HNE and PE, and HNE in sputum was assessed using colourimetric and fluorescent substrate assays by kinetic measurements, and by examining the interaction of HNE with inhibitors. Ceftazidime, tobramycin, and gentamycin slightly inhibited purified HNE activity whereas erythromycin and colistin significantly stimulated purified HNE and PE (395 and 557%, respectively). However, only colistin increased HNE activity in sputum (+102%) and was therefore studied in more detail. This increase in activity was not due an interference with the specific inhibition of HNE by α 1 -antitrypsin but colistin was found to reverse the inhibitory effects of small molecular weight molecules like heparin. Colistin increases the activity of human neutrophil elastase and Pseudomonas aeruginosa elastase, two proteases that contribute to the pathogenesis of cystic fibrosis airway disease. |
| Databáze: | OpenAIRE |
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