Application of 300× Enhanced Fluorescence on a Plasmonic Chip Modified with a Bispecific Antibody to a Sensitive Immunosensor
| Autor: | Takamitsu Hattori, Mitsuo Umetsu, Keiko Tawa, Izumi Kumagai, Hikaru Nakazawa |
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| Rok vydání: | 2013 |
| Předmět: |
Immunoassay
Silver Materials science biology Substrate (chemistry) Nanotechnology Carbocyanines Microfluidic Analytical Techniques Surface Plasmon Resonance Chip Fluorescence Antibodies ErbB Receptors Adsorption biology.protein Surface modification General Materials Science Soluble Epidermal Growth Factor Receptor Zinc Oxide Antibody Plasmon Fluorescent Dyes |
| Zdroj: | ACS Applied Materials & Interfaces. 5:8628-8632 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/am402173y |
| Popis: | The grating substrate covered with a metal layer, a plasmonic chip, and a bispecific antibody can play a key role in the sensitive detection of a marker protein with an immunosensor, because of the provision of an enhanced fluorescence signal and the preparation of a sensor surface densely modified with capture antibody, respectively. In this study, one of the tumor markers, a soluble epidermal growth factor receptor (sEGFR), was selected as the target to be detected. The ZnO- and silver-coated plasmonic chip with precise regularity and the appropriate duty ratio in the periodic structure further enhanced the fluorescence intensity. As for sensor surface modification with capture antibody, a bispecific antibody (anti-sEGFR and anti-ZnO antibody), the concentrated bispecific antibody solution was found to nonlinearly form a surface densely immobilized with antibody, because the binding process of a bispecific antibody to the ZnO surface can be a competitive process with adsorption of phosphate. As a result, the interface on the plasmonic chip provided a 300× enhanced fluorescence signal compared with that on a ZnO-coated glass slide, and therefore sEGFR was found to be quantitatively detected in a wide concentration range from 10 nM to 700 fM on our plasmonic surface. |
| Databáze: | OpenAIRE |
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