HPLC-ESI- MS/MS analysis of beet (Beta vulgaris) leaves and its beneficial properties in type 1 diabetic rats

Autor: Nesrine M. Hegazi, Aziz Yasri, Eman A. Abd El-Ghffar, Alaa Barakat, Hamada Saad, Mohamed Mostafa Soliman, Mohamed A El-Raey, Safia Mohamed Shehata, Mansour Sobeh
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Blood Glucose
Male
0301 basic medicine
Phytochemicals
Hplc esi ms ms
High-performance liquid chromatography
Antioxidants
Nuclear factor kappa B
chemistry.chemical_compound
0302 clinical medicine
Tandem Mass Spectrometry
Malondialdehyde
Alloxan
Chromatography
High Pressure Liquid
Chemistry
General Medicine
C-glycosyl flavonoids
Glutathione
Lipids
Mass spectrometric
Molecular Docking Simulation
Liver
030220 oncology & carcinogenesis
Molecular networking
Cytokines
Female
Inflammation Mediators
Beta vulgaris
Spectrometry
Mass
Electrospray Ionization
Electrospray ionization
RM1-950
Mass spectrometry
Diabetes Mellitus
Experimental
03 medical and health sciences
Phenols
Toxicity Tests
Acute
Animals
Humans
Rats
Wistar
Beta (finance)
Pharmacology
Chromatography
Plant Extracts
Body Weight
Atherosclerosis
PPAR gamma
Plant Leaves
PPAR-ɣ
Diabetes Mellitus
Type 1
030104 developmental biology
Therapeutics. Pharmacology
Phytotherapy
Zdroj: Biomedicine & Pharmacotherapy, Vol 120, Iss, Pp-(2019)
ISSN: 0753-3322
Popis: The phenolic profile of the leaves of Beta vulgaris subspecies vulgaris variety rubra was investigated by high-performance liquid chromatography (HPLC) coupled to electrospray ionization high resolution mass spectrometric (ESI-HRMS-MS) detection. Mass spectrometry-based molecular networking was employed to dereplicate the known compounds. Twelve known compounds, seven of which are previously undescribed as constituents in the B. vulgaris leaves were dereplicated and assigned with various levels of identification confidence. The ameliorative effects of the aqueous methanolic extract of the leaves were assessed against alloxan induced diabetic rats. It was found that the extract significantly decreased (p < 0.001) serum glucose, lipid profile, ALT, AST, TNF-α, IL-1β, IL-6, and hepatic MDA levels; and significantly increased (p
Databáze: OpenAIRE
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