Genetic characterization of Trypanosoma brucei circulating in domestic animals of the Fontem sleeping sickness of Cameroon
| Autor: | Guy Roger Njitchouang, Gérard Cuny, Gustave Simo, Flobert Njiokou, Tazoacha Asonganyi |
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| Rok vydání: | 2011 |
| Předmět: |
Veterinary medicine
Microsatellite markers Genotype Tsetse Flies Swine Immunology Trypanosoma brucei brucei Trypanosoma brucei Microbiology Dogs genotypes Trypanosomiasis medicine Polymorphic Microsatellite Marker Animals Cameroon Allele Sheep biology Goats Genetic Variation Sleeping sickness DNA Protozoan medicine.disease biology.organism_classification Blood meal Insect Vectors Blood film Infectious Diseases Microsatellite Multiple Microsatellite Repeats |
| Zdroj: | Microbes and infection. 14(7-8) |
| ISSN: | 1769-714X |
| Popis: | To improve our knowledge on the transmission dynamics of trypanosomes, Trypanosoma brucei was identified in domestic animals of the Fontem sleeping sickness focus of Cameroon and their genetic characterizations were performed using seven polymorphic microsatellite markers. About 397 domestic animals including 225 pigs, 87 goats, 65 sheep and 20 dogs were sampled. The card agglutination test for trypanosomiasis was positive for 254 (63.98%) animals while the parasitological examinations (thin blood film and capillary tube centrifugation) revealed 86 (21.66%) trypanosome infections. The PCR based method revealed 140 (35.26%) infections of trypanosomes of the subgenus Trypanozoon. The genetic characterization of these 140 positive samples revealed 89 different alleles: 82 in pigs, 72 in goat, 60 in sheep and 48 in dog. Whatever the microsatellite marker used, most of positive samples were amplified. However, the sensitivity (percentage of samples amplified for each marker) of these markers varies significantly between them (chi(2) = 120.32; P < 0.0001). This study showed a high level (80.00%) of mixed genotypes as well as a wide range of T brucei genotypes circulating in domestic animals of the Fontem sleeping sickness focus of Cameroon. This indicates that several T brucei genotypes can naturally be transmitted simultaneously to tsetse flies during a single blood meal. |
| Databáze: | OpenAIRE |
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