Environmental conditions during glycerol bioconversion affect 3‐hydroxypropionic acid bioproduction by Limosilactobacillus reuteri DSM 17938

Autor: Thi‐Lan‐Phuong Nguyen, Catherine Béal, Sarrah Ghorbal, Claire Saulou‐Bérion
Rok vydání: 2022
Předmět:
Zdroj: Biotechnology Progress. 39
ISSN: 1520-6033
8756-7938
DOI: 10.1002/btpr.3299
Popis: 3-Hydroxypropionic acid (3-HP) is a platform molecule whose biological production was carried out by the bacterium Limosilactobacillus reuteri according to a two-step process: first, a growth phase in batch mode on glucose, then a glycerol bioconversion into 3-HP in fed-batch mode. With the objective of improving 3-HP bioproduction, this study aimed at defining the operating conditions during the bioconversion phase that increases the bioproduction performance. A central composite rotatable design allowed testing various pH levels and specific glycerol feeding rates. By establishing response surfaces, optimal conditions have been identified that were different depending on the considered output variable (final 3-HP quantity, 3-HP production yield and production rate). Of them, 3-HP final quantity and 3-HP production yield were maximized at pH 6.0 and at specific glycerol feeding rates of 60 and 55 mg
Databáze: OpenAIRE
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