1,25-Dihydroxyvitamin D3 selectively translocates PKCalpha to nuclei in ROS 17/2.8 cells
| Autor: | Paul J. Bertics, Moisés A. Rivera-Bermúdez, Ralph M. Albrecht, Rashid Mosavin, William S. Mellon |
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| Rok vydání: | 2002 |
| Předmět: |
Bisindolylmaleimide
Protein Kinase C-alpha Time Factors Immunoblotting Osteocalcin Fluorescent Antibody Technique Gene Expression Bone Neoplasms Biology Biochemistry chemistry.chemical_compound Endocrinology Calcitriol Osteocalcin gene Tumor Cells Cultured Humans RNA Messenger Molecular Biology Protein kinase C Protein Kinase C Cell Nucleus Messenger RNA Osteosarcoma Pkc isoenzymes Blotting Northern Molecular biology Isoenzymes Calcium Channel Agonists chemistry Cell culture biology.protein Dactinomycin Tetradecanoylphorbol Acetate Rat Osteosarcoma Subcellular Fractions |
| Zdroj: | Molecular and cellular endocrinology. 188(1-2) |
| ISSN: | 0303-7207 |
| Popis: | We have investigated protein kinase C (PKC) regulation by 1,25-(OH) 2 D 3 in the rat osteosarcoma cell line ROS 17/2.8 since previous reports have implicated PKC in the 1,25-(OH) 2 D 3 -mediated regulation of osteocalcin gene expression (J. Biol. Chem. 267 (1992) 12562; Endocrinology 136 (1995) 5685). Here we report that 1,25-(OH) 2 D 3 increased PKCα, but not PKCβI, e or ζ, levels in the nuclear fraction in a time-dependent manner. Unlike PMA, 1,25-(OH) 2 D 3 did not alter the association of any of the expressed PKC isoenzymes with the plasma membrane. Treatment with 20 nM 1,25-(OH) 2 D 3 for 15 min, 30 min, 1 h and 24 h increased PKCα levels in the nuclear fraction by 2.3- to 2.6-fold. Nuclear PKCα expression was also increased with doses of 1,25-(OH) 2 D 3 as low as a 0.05 nM. 1,25-(OH) 2 D 3 -mediated stabilization of osteocalcin mRNA (Arch. Biochem. Biophys. 332 (1996) 142) was inhibited with bisindolylmaleimide treatment, suggesting that PKCα may be involved in the 1,25-(OH) 2 D 3 -mediated regulation of osteocalcin gene expression. |
| Databáze: | OpenAIRE |
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