Pravastatin attenuates atherosclerosis after myocardial infarction by inhibiting inflammatory Ly6C monocytosis in apolipoprotein E knockout mice
| Autor: | Liang Hu, Hongqi Zhang, Yang Ou, Xiaojin Liu, Jianguo Jia, Haiming Shi, Xinping Luo, Wei Shen, Shengjia Sun, Guomin Zhou, Yufei Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Apolipoprotein E medicine.medical_specialty Medicine (General) Inflammation Inflammatory monocyte 030204 cardiovascular system & hematology Biochemistry 03 medical and health sciences 0302 clinical medicine R5-920 Monocytosis Internal medicine medicine Myocardial infarction Monocyte subsets business.industry Biochemistry (medical) Cell Biology General Medicine medicine.disease 030104 developmental biology Endocrinology Knockout mouse lipids (amino acids peptides and proteins) medicine.symptom business Pravastatin medicine.drug |
| Zdroj: | Journal of International Medical Research, Vol 48 (2020) |
| ISSN: | 1473-2300 |
| Popis: | Objective To evaluate the protective effect of pravastatin on atherosclerotic development and inflammatory monocyte subset in atherosclerotic apolipoprotein E (ApoE)−/− mice after myocardial infarction (MI). Methods Male ApoE−/− mice (8 weeks old) were fed a high-fat diet for 14 weeks throughout the experiment. A MI model was produced using 18-week-old ApoE−/− mice. They were randomly divided into three groups: sham group, MI group, and MI+Pra group (40 mg/kg/day pravastatin). After 4 weeks (at the end of the study period), the mice were sacrificed and cardiac function was evaluated by echocardiography. Aortic lesion areas were evaluated using oil red O staining. Plaque macrophage in aortic sinus was analyzed by immunofluorescence staining. Flow cytometry was used to explore the proportions of monocyte subsets in the blood, spleen, and bone marrow． Results Pravastatin improved cardiac function and reduced lesion areas. It also attenuated the supply of monocytes in spleen, especially the inflammatory Ly6Chigh monocyte subset. Pravastatin also subsequently reduced macrophage accumulation in atherosclerotic lesions. Conclusions MI accelerated chronic atherosclerosis progress. Pravastatin suppressed atherosclerotic development and inhibited inflammatory monocytosis after MI in ApoE−/− mice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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