P-Glycoprotein expression in human retinal pigment epithelium cell lines
| Autor: | Diana E. M. Dolman, Geoffrey B. Arden, N. Joan Abbott, John G Lawrenson, Paul A Constable |
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| Rok vydání: | 2005 |
| Předmět: |
Cell type
Immunocytochemistry Biology Rhodamine 123 Cell Line Cellular and Molecular Neuroscience chemistry.chemical_compound Cytosol medicine Humans ATP Binding Cassette Transporter Subfamily B Member 1 RNA Messenger Eye Proteins Pigment Epithelium of Eye P-glycoprotein Retinal pigment epithelium Reverse Transcriptase Polymerase Chain Reaction Rhodamines Biological Transport Transfection Calcium Channel Blockers Molecular biology Immunohistochemistry Sensory Systems In vitro Ophthalmology medicine.anatomical_structure chemistry Verapamil Cell culture biology.protein Genes MDR |
| Zdroj: | Experimental eye research. 83(1) |
| ISSN: | 0014-4835 |
| Popis: | P-Glycoprotein (P-gp), an active efflux transporter encoded by the MDR1 gene, has recently been identified in the human and pig retinal pigment epithelium (RPE) in situ. Efflux pumps such as P-gp are major barriers to drug delivery in several tissues. We wished to establish whether human RPE cell lines express P-gp under the culture conditions recommended for each cell line so as to determine their suitability as in vitro models for predicting drug transport across the outer blood–retinal barrier. Three human RPE cell lines, ARPE19, D407 and h1RPE were investigated. Reverse transcriptase–polymerase chain reaction (RT-PCR) was carried out to determine the expression of MDR1 mRNA. Immunocytochemistry using the P-gp-specific antibody C219 was undertaken to investigate the presence of P-gp protein in each cell type. Uptake of rhodamine 123, a P-gp substrate, in the presence or absence of pre-treatment with a P-gp inhibitor, verapamil, was measured in each cell line to determine functional expression of P-gp. For all experiments, MDCK cells stably transfected with the human MDR1 gene (MDCK-MDR1) were used as a positive control. ARPE19 cells were consistently negative for P-gp as assessed by RT-PCR and immunocytochemistry. By contrast, RT-PCR of D407 and h1RPE samples yielded weak bands corresponding to MDR1; P-gp protein expression, as demonstrated by C219 immunoreactivity, was also present. Rhodamine uptake after treatment with verapamil was significantly greater in D407 and MDCK-MDR1, indicating functional expression of P-gp in these two cell lines. No evidence of functional P-gp was found in ARPE19 and h1RPE. In conclusion, D407 and h1RPE cells express P-gp, though functional activity was demonstrable only in D407 cells. ARPE19 cells do not express P-gp. Of these human RPE cells lines D407 could be considered as a suitable model for in vitro drug transport studies, particularly those involving P-gp substrates, without modification of their usual culture conditions. |
| Databáze: | OpenAIRE |
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