Expression and copy number analysis of TRPS1, EIF3S3 and MYC genes in breast and prostate cancer

Autor: Glenn T.G. Chang, Kimmo Savinainen, Teuvo L.J. Tammela, Tapio Visakorpi, Albert O. Brinkmann, Outi R. Saramäki, Marika J. Linja
Přispěvatelé: Developmental Biology
Rok vydání: 2004
Předmět:
Male
Cancer Research
Pathology
medicine.medical_specialty
Neoplasms
Hormone-Dependent

Eukaryotic Initiation Factor-3
Gene Dosage
Genes
myc

Prostatic Hyperplasia
Copy number analysis
Breast Neoplasms
MYC
Biology
Gene dosage
Prostate cancer
breast cancer
Breast cancer
TRPS1
SDG 3 - Good Health and Well-being
Prostate
Gene duplication
Tumor Cells
Cultured

medicine
Humans
RNA
Neoplasm

Copy-number variation
In Situ Hybridization
Fluorescence

EIF3S3
Oncogene
Reverse Transcriptase Polymerase Chain Reaction
Gene Amplification
Molecular and Cellular Pathology
Prostatic Neoplasms
DNA
Neoplasm

prostate cancer
medicine.disease
Neoplasm Proteins
DNA-Binding Proteins
Repressor Proteins
medicine.anatomical_structure
Oncology
Cancer research
Female
Neoplasm Recurrence
Local

DNA Probes
Chromosomes
Human
Pair 8

Transcription Factors
overexpression
Zdroj: British Journal of Cancer, 90, 1041-1046. Nature Publishing Group
British Journal of Cancer
ISSN: 0007-0920
Popis: The long arm of chromosome 8 is one of the most common regions of amplification in cancers of several organs, especially carcinomas of the breast and prostate. TRPS1, MYC and EIF3S3 genes are located in one of the minimal regions of amplification, 8q23-q24, and have been suggested to be the target genes of the amplification. Here, our goal was to study copy number and expression of the three genes in order to investigate the significance of the genes in breast and prostate cancer. By using fluorescence in situ hybridisation (FISH), we first found that TRPS1 and EIF3S3 were amplified together in about one-third of hormone-refractory prostate carcinomas. Next, we analysed the mRNA expression of the three genes by real-time quantitative RT-PCR and the gene copy number by FISH in six breast and five prostate cancer cell lines. Breast cancer cell line, SK-Br-3, which contained the highest copy number of all three genes, showed overexpression of only EIF3S3. Finally, the expression levels of TRPS1, EIF3S3 and MYC were measured in freshly frozen clinical samples of benign prostate hyperplasia (BPH), as well as untreated and hormone-refractory prostate carcinoma. The TRPS1 and MYC expression levels were similar in all prostate tumour groups, whereas EIF3S3 expression was higher (P=0.029) in prostate carcinomas compared to BPH. The data suggest that the expression of EIF3S3 is increased in prostate cancer, and that one of the mechanisms underlying the overexpression is the amplification of the gene.
Databáze: OpenAIRE