An alternative method for the synthesis of competitor RNA transcripts useful for specific detection and quantitation of dengue virus serotype 2 genome and replicative intermediate RNA
| Autor: | Jose-Carmen Gudino, Yolanda González-Hernández, Abraham Landa-Piedra, Alejandro Escobar-Gutiérrez, Hiram Olivera, Gilberto Vaughan |
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| Rok vydání: | 2007 |
| Předmět: |
viruses
RNA-dependent RNA polymerase Genome Viral Biology Dengue virus medicine.disease_cause Genome Sensitivity and Specificity Virus Cell Line Dengue Virology medicine Animals Humans Serotyping Gene Messenger RNA Reverse Transcriptase Polymerase Chain Reaction RNA Dengue Virus biology.organism_classification Molecular biology Flavivirus RNA Viral |
| Zdroj: | Journal of virological methods. 152(1-2) |
| ISSN: | 0166-0934 |
| Popis: | The development of a quantitative-competitive reverse transcription-PCR (RT-PCR) assay to quantify dengue virus (DEN) genome (vRNA) and its replicative intermediate RNA (vRI) is described. A highly conserved region located on the DEN capsid-premembrane genes was used to produce a competitor RNA molecule which contains an internal deletion of 70 nucleotides. The competitor provides a suitable internal control useful to quantify viral RNA from all four dengue virus (DEN 1-4) serotypes. The detection limit of the assay was found to be 100 copies per reaction. This is a rapid, simple, sensitive, inexpensive and easy method for quantitation of DEN RNA species. |
| Databáze: | OpenAIRE |
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