Differential Control of Interleukin-6 mRNA Levels by Cellular Distribution of YB-1

Autor: Taeyun A. Lee, Sujin Kang, Eun A. Ra, Boyoun Park, Sungwook Lee, Eun Hye Lee, Hyun jin Choi
Rok vydání: 2014
Předmět:
Lipopolysaccharides
Cell
lcsh:Medicine
RNA-binding proteins
Biochemistry
Mice
RNA interference
Medicine and Health Sciences
Luciferases
lcsh:Science
Innate Immune System
Multidisciplinary
Reverse Transcriptase Polymerase Chain Reaction
Acetylation
Flow Cytometry
Protein Transport
medicine.anatomical_structure
Cytokines
RNA Interference
Intracellular
Research Article
Immunology
Enzyme-Linked Immunosorbent Assay
Biology
Real-Time Polymerase Chain Reaction
Cell Line
P-bodies
Extracellular
medicine
Animals
Humans
Secretion
RNA
Messenger
DNA Primers
Messenger RNA
Biology and life sciences
Interleukin-6
lcsh:R
Proteins
Dendritic Cells
Molecular Development
RNA stability
Molecular biology
Mice
Inbred C57BL
Microscopy
Fluorescence
Cytoplasm
Immune System
RNA
lcsh:Q
Y-Box-Binding Protein 1
RNA transport
Extracellular Space
Developmental Biology
Zdroj: PLoS ONE, Vol 9, Iss 11, p e112754 (2014)
PLoS ONE
PLOS ONE(9): 11
ISSN: 1932-6203
Popis: Cytokine production is essential for innate and adaptive immunity against microbial invaders and must be tightly controlled. Cytokine messenger RNA (mRNA) is in constant flux between the nucleus and the cytoplasm and in transcription, splicing, or decay; such processes must be tightly controlled. Here, we report a novel function of Y-box-binding protein 1 (YB-1) in modulating interleukin-6 (IL-6) mRNA levels in a cell type-specific manner. In lipopolysaccharide (LPS)-stimulated macrophages, YB-1 interacts with IL-6 mRNA and actively transports it to the extracellular space by YB-1-enriched vesicles, resulting in the proper maintenance of intracellular IL-6 mRNA levels. YB-1 secretion occurs in a cell type-specific manner. Whereas macrophages actively secret YB-1, dendritic cells maintain it predominantly in the cytoplasm even in response to LPS. Intracellular YB-1 has the distinct function of regulating IL-6 mRNA stability in dendritic cells. Moreover, because LPS differentially regulates the expression of histone deacetylase 6 (HDAC6) in macrophages and dendritic cells, this stimulus might control YB-1 acetylation differentially in both cell types. Taken together, these results suggest a unique feature of YB-1 in controlling intracellular IL-6 mRNA levels in a cell type-specific manner, thereby leading to functions that are dependent on the extracellular and intracellular distribution of YB-1.
Databáze: OpenAIRE
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