Differential Control of Interleukin-6 mRNA Levels by Cellular Distribution of YB-1
Autor: | Taeyun A. Lee, Sujin Kang, Eun A. Ra, Boyoun Park, Sungwook Lee, Eun Hye Lee, Hyun jin Choi |
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Rok vydání: | 2014 |
Předmět: |
Lipopolysaccharides
Cell lcsh:Medicine RNA-binding proteins Biochemistry Mice RNA interference Medicine and Health Sciences Luciferases lcsh:Science Innate Immune System Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction Acetylation Flow Cytometry Protein Transport medicine.anatomical_structure Cytokines RNA Interference Intracellular Research Article Immunology Enzyme-Linked Immunosorbent Assay Biology Real-Time Polymerase Chain Reaction Cell Line P-bodies Extracellular medicine Animals Humans Secretion RNA Messenger DNA Primers Messenger RNA Biology and life sciences Interleukin-6 lcsh:R Proteins Dendritic Cells Molecular Development RNA stability Molecular biology Mice Inbred C57BL Microscopy Fluorescence Cytoplasm Immune System RNA lcsh:Q Y-Box-Binding Protein 1 RNA transport Extracellular Space Developmental Biology |
Zdroj: | PLoS ONE, Vol 9, Iss 11, p e112754 (2014) PLoS ONE PLOS ONE(9): 11 |
ISSN: | 1932-6203 |
Popis: | Cytokine production is essential for innate and adaptive immunity against microbial invaders and must be tightly controlled. Cytokine messenger RNA (mRNA) is in constant flux between the nucleus and the cytoplasm and in transcription, splicing, or decay; such processes must be tightly controlled. Here, we report a novel function of Y-box-binding protein 1 (YB-1) in modulating interleukin-6 (IL-6) mRNA levels in a cell type-specific manner. In lipopolysaccharide (LPS)-stimulated macrophages, YB-1 interacts with IL-6 mRNA and actively transports it to the extracellular space by YB-1-enriched vesicles, resulting in the proper maintenance of intracellular IL-6 mRNA levels. YB-1 secretion occurs in a cell type-specific manner. Whereas macrophages actively secret YB-1, dendritic cells maintain it predominantly in the cytoplasm even in response to LPS. Intracellular YB-1 has the distinct function of regulating IL-6 mRNA stability in dendritic cells. Moreover, because LPS differentially regulates the expression of histone deacetylase 6 (HDAC6) in macrophages and dendritic cells, this stimulus might control YB-1 acetylation differentially in both cell types. Taken together, these results suggest a unique feature of YB-1 in controlling intracellular IL-6 mRNA levels in a cell type-specific manner, thereby leading to functions that are dependent on the extracellular and intracellular distribution of YB-1. |
Databáze: | OpenAIRE |
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