Potency of isothiocyanates to induce luciferase reporter gene expression via the electrophile-responsive element from murine glutathione S-transferase Ya

Autor: Ivonne M.C.M. Rietjens, Jac M.M.J.G. Aarts, Barry M.G. Blankvoort, Wouter H. J. Vaes, Martijn Vermeulen, Peter J. van Bladeren, Anne-Marie J. F. Boerboom
Přispěvatelé: TNO Kwaliteit van Leven
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Phenethyl isothiocyanate
enzyme induction
sulforaphane
animal cell
Toxicology
chemistry.chemical_compound
Mice
Isothiocyanate
Genes
Reporter
Isothiocyanates
Gene expression
glutathione transferase
mercapturic acids
Electrophile-responsive element
phenyl isothiocyanate
Luciferases
Glutathione Transferase
risk
Regulation of gene expression
biology
article
General Medicine
gene expression regulation
luciferase
reporter gene
cell assay
unclassified drug
gene induction
Glutathione S-transferase
isothiocyanic acid derivative
Biochemistry
consensus sequence
Antioxidant
drug potency
vegetables
Cell Survival
enzymes
mechanism
Response Elements
electrophile
Cell Line
Animals
Luciferase
controlled study
Enzyme inducer
Biology
Analytical research
mouse
Toxicologie
gene location
VLAG
Reporter gene
nonhuman
fruit
Molecular biology
glutathione s transferase Ya
chemistry
Gene Expression Regulation
concentration response
biology.protein
chemical structure
identification
activation
Murinae
Zdroj: Toxicology in Vitro 23 (2009) 4
Toxicology in Vitro, 4, 23, 617-621
Toxicology in Vitro, 23(4), 617-621
ISSN: 0887-2333
Popis: Isothiocyanates are electrophiles that are able to induce phase II biotransformation enzyme gene expression via an electrophile-responsive element (EpRE) in the gene regulatory region. To study the potency of different isothiocyanates to induce the expression of EpRE-regulated genes, a Hepa-1c1c7 luciferase reporter cell line was exposed to structurally different isothiocyanates. The reporter cell line, EpRE(mGST-Ya)-LUX, contains the EpRE from the regulatory region of the mouse glutathione S-transferase Ya gene. Isothiocyanates containing a methyl-sulfur side chain, e.g. sulforaphane, showed a lower EC50 (0.8-3.2 μM) and a comparable induction factor (17-22.4) compared to the structurally different isothiocyanates containing an alkyl or aromatic side chain, e.g. allyl and phenylethyl isothiocyanate (EC50 3.9-6.5 μM, induction factor 17.5-23). After 24 h of exposure, on average (±SD) 23 ± 5% of the isothiocyanate was found in the cells and 77% in the cell medium. Isothiocyanates prove to be strong inducers of electrophile-responsive element-mediated gene expression at physiological concentrations. The here described luciferase reporter cell line is a suitable assay to measure the potency of compounds to induce EpRE-regulated gene expression. © 2009 Elsevier Ltd. All rights reserved.
Databáze: OpenAIRE
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