Mapping astrocyte activity domains by light sheet imaging and spatio-temporal correlation screening
| Autor: | Dongdong Li, Daniela Herrera Moro, Claire Martin, Christine Mouffle, Cuong Pham, Steve Didienne, Régine Hepp, Frank W. Pfrieger, Pascal Legendre, Jean-Marie Mangin, Serge Luquet, Bruno Cauli |
|---|---|
| Přispěvatelé: | Neurosciences Paris Seine (NPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Biologie Paris Seine (IBPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut des Neurosciences Cellulaires et Intégratives (INCI), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2020 |
| Předmět: |
Optical sectioning
Cognitive Neuroscience [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Cell GCaMP 050105 experimental psychology Fluorescence lcsh:RC321-571 03 medical and health sciences Mice 0302 clinical medicine medicine Fluorescence microscope Biological neural network Animals 0501 psychology and cognitive sciences Calcium Signaling lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry G protein-coupled receptor Neurons Chemistry 05 social sciences Brain medicine.anatomical_structure Neurology Microscopy Fluorescence Light sheet fluorescence microscopy Astrocytes Calcium Neuroscience 030217 neurology & neurosurgery Astrocyte |
| Zdroj: | NeuroImage NeuroImage, Elsevier, 2020, 220, pp.117069. ⟨10.1016/j.neuroimage.2020.117069⟩ NeuroImage, Vol 220, Iss, Pp 117069-(2020) |
| ISSN: | 1095-9572 1053-8119 |
| DOI: | 10.1016/j.neuroimage.2020.117069⟩ |
| Popis: | International audience; Astrocytes are a major type of glial cell in the mammalian brain, essentially regulating neuronal development and function. Quantitative imaging represents an important approach to study astrocytic signaling in neural circuits. Focusing on astrocytic Ca 2þ activity, a key pathway implicated in astrocye-neuron interaction, we here report a strategy combining fast light sheet fluorescence microscopy (LSFM) and correlative screening-based time series analysis, to map activity domains in astrocytes in living mammalian nerve tissue. Light sheet of micron-scale thickness enables wide-field optical sectioning to image astrocytes in acute mouse brain slices. Using both chemical and genetically encoded Ca 2þ indicators, we demonstrate the complementary advantages of LSFM in mapping Ca 2þ domains in astrocyte populations as compared to epifluorescence and two-photon microscopy. Our approach then revealed distinct kinetics of Ca 2þ signals between cortical and hypothalamic astrocytes in resting conditions and following the activation of adrenergic G protein coupled receptor (GPCR). This observation highlights the activity heterogeneity across regionally distinct astrocyte populations, and indicates the potential of our method for investigating dynamic signals in astrocytes. |
| Databáze: | OpenAIRE |
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