R-Ras1 and R-Ras2 Expression in Anatomical Regions and Cell Types of the Central Nervous System
| Autor: | Gonzalo Garcia-Martin, Miriam Sanz-Rodriguez, Berta Alcover-Sanchez, Marta P. Pereira, Francisco Wandosell, Beatriz Cubelos |
|---|---|
| Přispěvatelé: | UAM. Departamento de Biología Molecular |
| Rok vydání: | 2022 |
| Předmět: |
Central Nervous System
Male QH301-705.5 myelin R-Ras optic nerve neurodegenerative diseases neuron Optic nerve Article Catalysis Inorganic Chemistry Gene Knockout Techniques Mice Animals Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Myelin Sheath Spectroscopy Monomeric GTP-Binding Proteins Neurons Neurodegenerative diseases Organic Chemistry Membrane Proteins General Medicine Neuron Biología y Biomedicina / Biología Up-Regulation Computer Science Applications Chemistry Myelin Organ Specificity Astrocytes ras Proteins Female Microglia |
| Zdroj: | International Journal of Molecular Sciences Biblos-e Archivo. Repositorio Institucional de la UAM instname International Journal of Molecular Sciences; Volume 23; Issue 2; Pages: 978 International Journal of Molecular Sciences, Vol 23, Iss 978, p 978 (2022) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms23020978 |
| Popis: | Since the optic nerve is one of the most myelinated tracts in the central nervous system (CNS), many myelin diseases affect the visual system. In this sense, our laboratory has recently reported that the GTPases R-Ras1 and R-Ras2 are essential for oligodendrocyte survival and maturation. Hypomyelination produced by the absence of one or both proteins triggers axonal degeneration and loss of visual and motor function. However, little is known about R-Ras specificity and other possible roles that they could play in the CNS. In this work, we describe how a lack of R-Ras1 and/or R-Ras2 could not be compensated by increased expression of the closely related R-Ras3 or classical Ras. We further studied R-Ras1 and R-Ras2 expression within different CNS anatomical regions, finding that both were more abundant in less-myelinated regions, suggesting their expression in non-oligodendroglial cells. Finally, using confocal immunostaining colocalization, we report for the first time that R-Ras2 is specifically expressed in neurons. Neither microglia nor astrocytes expressed R-Ras1 or R-Ras2. These results open a new avenue for the study of neuronal R-Ras2’s contribution to the process of myelination. |
| Databáze: | OpenAIRE |
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