Applicability of Brugia malayi immune antibody library for the isolation of a human recombinant monoclonal antibody to Echinococcus granulosus antigen B
| Autor: | Rahmah Noordin, Azimah Ahmad, Jing Yi Lai, Zamrina Baharudeen, Anizah Rahumatullah, Theam Soon Lim |
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| Rok vydání: | 2020 |
| Předmět: |
Phage display
medicine.drug_class Lipoproteins Blotting Western Immunology Antibodies Helminth Enzyme-Linked Immunosorbent Assay Monoclonal antibody Brugia malayi law.invention Epitopes Immune system Antigen Echinococcosis law parasitic diseases medicine Animals Humans Echinococcus granulosus Base Sequence biology Antibodies Monoclonal General Medicine biology.organism_classification Virology Infectious Diseases biology.protein Recombinant DNA Electrophoresis Polyacrylamide Gel Parasitology Antibody |
| Zdroj: | Experimental Parasitology. 219:108029 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2020.108029 |
| Popis: | Echinococcus granulosus is a worldwide zoonotic infection that causes human cystic echinococcosis (CE) or hydatid disease. The present study describes the isolation and production of a monoclonal antibody against recombinant AgB protein using the developed Human AntibodY Disease ENhanced (HAYDEN)-Filariasis library. The DNA sequences of the isolated clones were analyzed, followed by gene analysis and binding assays. Clone E1 showed a full-length sequence and represents the IgHV5-LV3 antibody gene family. The antibody protein yield was satisfactory, and it reacted specifically against rAgB. The novel E1 protein is potentially useful for the development of an antigen detection assay for CE. The ability of the Brugia malayi immune antibody library to isolate antibodies against Echinococcus granulosus antigens highlights the broad coverage of immune antibody libraries. |
| Databáze: | OpenAIRE |
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