Total body irradiation tremendously impair the proliferation, differentiation and chromosomal integrity of bone marrow-derived mesenchymal stromal stem cells
Autor: | Chiao Lin Lin, Yu Chien Chang, Ji An Liang, Long Yuan Li, Chen Yuan Lin, Li Yuan Bai, Chang Fang Chiu, Chuan-Mu Chen, Wen Jyi Lo, Su-Peng Yeh, Ling Min Chao |
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Rok vydání: | 2018 |
Předmět: |
Adult
Male 0301 basic medicine China medicine.medical_specialty Transplantation Conditioning Stromal cell medicine.medical_treatment Apoptosis Bone Marrow Cells Chromosome Disorders Hematopoietic stem cell transplantation Hospitals University Necrosis Young Adult 03 medical and health sciences Internal medicine Tumor Cells Cultured medicine Humans Radiation Injuries Cells Cultured Cell Proliferation Chromosome Aberrations Leukemia Hematology business.industry Mesenchymal stem cell Hematopoietic Stem Cell Transplantation Cell Differentiation Mesenchymal Stem Cells General Medicine Total body irradiation Hematopoietic Stem Cells Adult Stem Cells 030104 developmental biology medicine.anatomical_structure Cancer research Female Bone marrow Stem cell business Whole-Body Irradiation Ex vivo |
Zdroj: | Annals of Hematology. 97:697-707 |
ISSN: | 1432-0584 0939-5555 |
Popis: | Total body irradiation (TBI) is frequently used in hematopoietic stem cell transplantation (HSCT) and is associated with many complications due to radiation injury to the normal cells, including normal stem cells. Nevertheless, the effects of TBI on the mesenchymal stromal stem cell (MSC) are not fully understood. Bone marrow-derived MSCs (BM-MSCs) isolated from normal adults were irradiated with 200 cGy twice daily for consecutive 3 days, a regimen identical to that used in TBI-conditioning HSCT. The characteristics, differentiation potential, cytogenetics, hematopoiesis-supporting function, and carcinogenicity of the irradiated BM-MSCs were then compared to the non-irradiated control. The irradiated and non-irradiated MSCs shared similar morphology, phenotype, and hematopoiesis-supporting function. However, irradiated MSCs showed much lower proliferative and differentiative potential. Irradiation also induced clonal cytogenetic abnormalities of MSCs. Nevertheless, the carcinogenicity of irradiated MSCs is low in vitro and in vivo. In parallel with the ex vivo irradiation experiments, decreased proliferative and differentiative abilities and clonal cytogenetic abnormalities can also be found in MSCs isolated from transplant recipients who had received TBI-based conditioning previously. Thus, TBI used in HSCT drastically injury MSCs and may contribute to the development of some long-term complications associated with clonal cytogenetic abnormality and poor adipogenesis and osteogenesis after TBI. |
Databáze: | OpenAIRE |
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