DMSO mimics inhibitory effect of thalidomide on choriocapillary endothelial cell proliferation in culture
| Autor: | M Spitznas, Nicole Eter |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Endothelium
Cell division Angiogenesis Inhibitors Biology Immunoenzyme Techniques Cellular and Molecular Neuroscience chemistry.chemical_compound medicine Animals Dimethyl Sulfoxide Cells Cultured Choroid Dimethyl sulfoxide Cell growth Trypsin Molecular biology Sensory Systems Capillaries Thalidomide Endothelial stem cell Laboratory Science Ophthalmology medicine.anatomical_structure Microscopy Fluorescence Biochemistry chemistry Cell culture Cattle Cell Division medicine.drug |
| Zdroj: | British Journal of Ophthalmology. 86:1303-1305 |
| ISSN: | 0007-1161 |
| Popis: | Aim: To evaluate the effect of thalidomide on bovine choriocapillary endothelial cell proliferation. Methods: Posterior segments of bovine eyes were incubated with trypsin. After digestion of the tapetum, pieces of Bruch’s membrane with the adherent choriocapillaris were scraped, digested again with a cocktail of enzymes, washed and cultured in MCDB 131 medium. Cells were incubated with 100 μg/ml thalidomide (dissolved in DMSO as recommended). Control cultures were incubated with DMSO alone or without any supplement. Cell proliferation after 24 hours was determined in a counting chamber. Purity of the cultures was controlled by immunohistochemical staining and labelling with low density lipoprotein. Results: Thalidomide dissolved in DMSO inhibited proliferation of choriocapillary endothelial cells compared to control cultures incubated without any supplement. However, DMSO alone suppressed cell proliferation equally well. Conclusion: In cultured bovine choriocapillary cells the inhibiting effect of thalidomide may at least in part be attributed to the solvent DMSO alone. |
| Databáze: | OpenAIRE |
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