Histomorphologic and ultrastructural recovery of myopathy in rats treated with low-level laser therapy
| Autor: | David Cremonezzi, Vilma Campana, Natalia Servetto, Juan Carlos Simes, Antonio Di Pietro |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Pathology
medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD medicine.medical_treatment Inmunología Strain (injury) Inflammation Dermatology Mitochondrion Mitochondrial alteration MYOPATHY 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Muscular Diseases INFLAMMATION MITOCHONDRIA medicine Animals Low-Level Light Therapy Muscle Skeletal GALLIUM-ARSENIDE LASER Myopathy Low level laser therapy LLLT business.industry 030206 dentistry medicine.disease Muscle Striated Mitochondria Rats Medicina Básica Lasers Gas Ultrastructure Female HELIUM-NEON LASER Surgery Lasers Semiconductor medicine.symptom Myofibril business |
| Zdroj: | Lasers in Medical Science. 32:841-849 |
| ISSN: | 1435-604X 0268-8921 |
| Popis: | The purpose of the present work was to study the effect of low-level laser therapy (LLLT): helium-neon (He-Ne) and gallium arsenide (Ga-As) laser on the histomorphology of muscle and mitochondria in experimental myopathy in rats. Thirty Suquía strain female rats were distributed in groups: (A) control (intact), (B) injured, (C) injured and treated with He-Ne laser, (D) injured and treated with Ga-As laser, (E) irradiated with He-Ne laser on the non-injured muscle, and (F) irradiated with Ga-As laser on the non-injured muscle. Myopathy was induced by injecting 0.05 mg/rat/day of adrenaline in the left gastrocnemius muscle at the same point on five consecutive days, in groups B, C, and D. LLLT was applied with 9.5 J cm−2 daily for seven consecutive days in groups C, D, E, and F. The muscles were examined with optic and electronic microscopy. The inflammation was classified as absent, mild, and intense and the degree of mitochondrial alteration was graded I, II, III, and IV. Categorical data were statistically analyzed by Chi-square and the Fisher-Irwin Bilateral test, setting significant difference at p < 0.05. The damage found in muscle and mitochondria histomorphology in animals with induced myopathy (B) was intense or severe inflammation with grade III or IV of mitochondrial alteration. They underwent significant regression (p < 0.001) compared with the groups treated with He-Ne (C) and Ga-As (D) laser, in which mild or moderate inflammation was seen and mitochondrial alteration grades I and II, recovering normal myofibrillar architecture. No differences were found between the effects caused by the two lasers, or between groups A, E, and F. Group A was found to be different from B, C, and D (p < 0.001). LLLT in experimental myopathy caused significant muscular and mitochondrial morphologic recovery. Fil: Servetto, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina Fil: Cremonezzi, David César. Universidad Nacional de La Rioja; Argentina. Universidad Nacional de Córdoba; Argentina Fil: Simes, Juan Carlos. Universidad Nacional de Córdoba; Argentina Fil: Di Pietro, Antonio. Universidad Nacional de Córdoba; Argentina Fil: Campana, Vilma. Universidad Nacional de Córdoba; Argentina |
| Databáze: | OpenAIRE |
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