A streptavidin functionalized graphene oxide/Au nanoparticles composite for the construction of sensitive chemiluminescent immunosensor
| Autor: | Juan Li, Dionysios D. Dionysiou, Shufen Luo, Xiaoya Hu, Suhua Yu, Juan Shen, Zhanjun Yang |
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| Rok vydání: | 2013 |
| Předmět: |
Detection limit
Streptavidin Immunoassay Luminescence Chemistry Nanoparticle Nanotechnology Biocompatible Materials Enzyme-Linked Immunosorbent Assay Oxides Biosensing Techniques Biochemistry Analytical Chemistry Contact angle chemistry.chemical_compound Linear range Colloidal gold Biotinylation Dielectric Spectroscopy Microscopy Electron Scanning Environmental Chemistry Graphite Biosensor Spectroscopy |
| Zdroj: | Analytica chimica acta. 839 |
| ISSN: | 1873-4324 |
| Popis: | In this work, a novel streptavidin functionalized graphene oxide/Au nanoparticles (streptavidin/GO/AuNPs) composite is prepared and for the first time used to construct sensitive chemiluminescent immunosensor for the detection of tumor marker. The streptavidin/GO/AuNPs composite and the immunosensor are characterized using scanning electron microscopy, static water contact angle measurement and electrochemical impedance spectroscopy. The biofunctionalized composite has large reactive surface area and excellent biocompatibility, thus the capture antibody can be efficiently immobilized on its surface based on the highly selective recognition of streptavidin to biotinylated antibody. Using α-fetoprotein (AFP) as a model, the proposed chemiluminescent immunosensor shows a wide linear range from 0.001 to 0.1 ng mL −1 with an extremely low detection limit down to 0.61 pg mL −1 . The resulting AFP immunosensor shows high detection sensitivity, fast assay speed, acceptable detection and fabrication reproducibility, good specificity and stability. The assay results of serum samples with the proposed method are in an acceptable agreement with the reference values. This work provides a promising biofunctionalized nanostructure for sensitive biosensing applications. |
| Databáze: | OpenAIRE |
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