Quiescence and proliferative response of normal human embryonic fibroblasts in homologous environment. Effect of aging
| Autor: | D Kletsas, D Stathakos |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Senescence
medicine.medical_treatment Fibroblast growth factor Culture Media Serum-Free medicine Humans Fibroblast Interphase Cells Cultured Cellular Senescence Platelet-Derived Growth Factor biology Epidermal Growth Factor Cell growth Growth factor Cell Biology Fibroblasts Embryo Mammalian Embryonic stem cell Cell biology Culture Media Fibroblast Growth Factors medicine.anatomical_structure Blood Cell culture Immunology biology.protein Platelet-derived growth factor receptor Cell Division |
| Zdroj: | Cell biology international reports. 16(2) |
| ISSN: | 0309-1651 |
| Popis: | A cell culture system for the study of human serum growth factors is described. It is based on a diploid human embryonic lung fibroblast cell strain (Flow 2002) with a finite life span (60±3 CPDS). When maintained in homologous (human) plasma-derived serum (PDS) at concentrations as low as 0.05%, the cells attain quiescence. Under these conditions, they remain viable for at least 14 days and they readily respond when stimulated to proliferate by human serum or human PDS, as well as by growth factors, such as PDGF, EGF, FGF. During in vitro aging, the cells retain their responsiveness to these growth stimuli, although the net proliferative effect decreases as they approach senescence. |
| Databáze: | OpenAIRE |
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