| Autor: |
R. M. Hochster, S. M. Lesley |
| Rok vydání: |
1959 |
| Předmět: |
|
| Zdroj: |
Canadian Journal of Biochemistry and Physiology. 37:513-529 |
| ISSN: |
0576-5544 |
| DOI: |
10.1139/y59-055 |
| Popis: |
The extracellular polysaccharide produced by Xanthomonas phaseoli has been purified and shown to be homogeneous by examination in the analytical ultra-centrifuge, by moving boundary electrophoresis, and by gradient precipitation with ethanol. Following hydrolysis the constituent sugars of the polysaccharide were isolated and positively identified as the crystalline derivatives of D-glucose and of D-mannose and as the crystalline lactone of D-glucuronic acid. The above hexoses were found to be present in the relative proportions of 1:1:1.The results of a preliminary investigation in which C14-labelled glucose was supplied to rapidly growing cells indicate that it is unlikely that a single six-carbon precursor is formed from which all three sugar components of the polysaccharide are synthesized directly. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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