Human desmoid fibroblasts: matrix metalloproteinases, their inhibitors and modulation by Toremifene
| Autor: | Alessio Becchetti, Chiara Balducci, Cinzia Lilli, Giammario Giustozzi, Giordano Stabellini, Paola Locci, Lucio Cagini, Lorella Marinucci |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Antineoplastic Agents Hormonal Matrix metalloproteinase inhibitor Biology Matrix metalloproteinase Matrix Metalloproteinase Inhibitors lcsh:RC254-282 Extracellular matrix Western blot Cell Line Tumor Genetics medicine Tumor Cells Cultured Humans Zymography Toremifene Collagenases medicine.diagnostic_test Tissue Inhibitor of Metalloproteinases Fibroblasts lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Matrix Metalloproteinases Up-Regulation body regions Procollagen peptidase Fibromatosis Aggressive Oncology Cancer research Collagenase Collagen Procollagen medicine.drug Research Article |
| Zdroj: | BMC Cancer, Vol 5, Iss 1, p 22 (2005) BMC Cancer |
| ISSN: | 1471-2407 |
| Popis: | Background Desmoid tumour is a benign, non metastasising neoplasm characterised by an elevated deposition of organic macromolecules in the extracellular matrix (ECM). The matrix metalloproteinases (MMPs) are a family of zinc-dependent proteinases involved in the degradation of ECM macromolecules. The MMPs and their natural inhibitors (TIMPs) have been implicated in tumour growth, invasion and metastasis. In this study we provide evidence that the in vitro cultured cell line from desmoid tumour accumulates more collagen fibres in the ECM than healthy fibroblasts. Methods We investigated collagen accumulation by 3H-thymidine incorporation, MMP expression by substrate gel zymography and TIMP expression by Western blot analysis. Results Desmoid fibroblasts showed a reduction in MMP activity and an increase of type I and III collagen and TIMPs compared to normal fibroblasts. Conclusion The increase in collagen in desmoid fibroblasts was due to inhibited collagen degradation (reduction of MMP activity) rather than to increased collagen synthesis. Adding toremifene, an anti-estrogen triphenylethylene derivate, to desmoid fibroblasts reduced collagen accumulation by decreasing mRNA expression and increasing collagen degradation. |
| Databáze: | OpenAIRE |
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