Phosphorylated transducer and activator of transcription-3 (pSTAT3) immunohistochemical expression in paired primary and metastatic colorectal cancer
| Autor: | Allen M. Gown, Daniel W. Yokom, Rachel Anne Goodwin, Derek J. Jonker, Justin Bateman, Horia Marginean, Esmeralda C. Marginean, Manijeh Daneshmand, Timothy R. Asmis, Shelly Sud, Joanna Gotfrit |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cancer Research Pathology medicine.medical_specialty Liver tumor Colorectal cancer Rectum lcsh:RC254-282 Metastasis 03 medical and health sciences Phosphorylated signal transducer and activator of transcription-3 (pSTAT3) 0302 clinical medicine Biopsy Primary cancer paired with liver metastasis medicine STAT3 Original Research medicine.diagnostic_test biology business.industry Clinical decisions medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Primary tumor Immunohistochemistry 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis biology.protein business |
| Zdroj: | Translational Oncology, Vol 14, Iss 2, Pp 100996-(2021) Translational Oncology |
| ISSN: | 1936-5233 |
| Popis: | Highlights • STAT3 regulates genes for cell growth and differentiation, inflammation, neoplasia. • STAT3 is a potential therapeutic target in colorectal cancer. • Most liver metastases stain STAT3 positive. • Discordant pSTAT3 expression in paired primary tumors and liver metastases. • Test on archived or a fresh liver biopsy. Background Signal Transducer and Activator of Transcription-3 (STAT3) mediates cellular functions. We assessed the IHC expression of phosphorylated STAT3 (pSTAT3) in paired primary tumors and liver metastases in patients with advanced stage colorectal cancer (CRC). Methods We included patients with tissue blocks available from both the primary CRC and a surgically resected liver metastasis. The IHC pSTAT3 expression agreement was measured using Cohen's kappa statistic. Results The study included 103 patients, 55% male, median age was 64. 43% tumors originated in rectum, and 63% of the primary tumors were synchronous. Expression of pSTAT3 was 76% in liver metastases and 71% in primary tumors. A difference in pSTAT3 staining between the primary tumor and liver metastases was noted in 64%. There was lost expression of pSTAT3 in the liver metastases in 28% and gained expression in 36% of cases compared to the primary. The kappa statistic comparing agreement between staining patterns of the primary tumors and liver metastases was a “less-than-chance”, at -0.02. Median survival was 4.9 years, with no difference in survival outcomes by pSTAT3 expression in the primary tumor or liver metastases. Discussion STAT3 is not a prognostic marker in the selective setting of metastatic CRC to liver, but it may remain a potential therapeutic target given most liver metastases expressed pSTAT3. Discordant pSTAT3 expression in between primary tumors and paired liver metastases suggests that use of this class of drug to treat liver predominant metastatic colorectal cancer in a biomarker-driven approach may require confirmatory liver tumor biopsy. |
| Databáze: | OpenAIRE |
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