Detection and confirmation of serum lipid biomarkers for preeclampsia using direct infusion mass spectrometry
Autor: | Sydney A Young, Bruce J. Jackson, H. Dennis Tolley, Benjamin Peaden, Swati Anand, T. Flint Porter, Michael W. Varner, Mary E. D'Alton, Sean Esplin, Steven W. Graves |
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Rok vydání: | 2016 |
Předmět: |
Adult
0301 basic medicine diagnosis Physiology QD415-436 030204 cardiovascular system & hematology Biochemistry Mass Spectrometry Preeclampsia lipids 03 medical and health sciences 0302 clinical medicine Endocrinology Pre-Eclampsia Lipidomics medicine Humans Blood Chemical Analysis Pregnancy Chromatography business.industry Reproducibility of Results Cell Biology medicine.disease Predictive value Pathophysiology 030104 developmental biology lipidomics Gestation Female mass spectrometer pregnancy Patient-Oriented and Epidemiological Research Lipid biomarkers business Biomarkers |
Zdroj: | Journal of Lipid Research, Vol 57, Iss 4, Pp 687-696 (2016) |
ISSN: | 0022-2275 |
Popis: | Despite substantial research, the early diagnosis of preeclampsia remains elusive. Lipids are now recognized to be involved in regulation and pathophysiology of some disease. Shotgun lipidomic studies were undertaken to determine whether serum lipid biomarkers exist that predict preeclampsia later in the same in pregnancy. A discovery study was performed using sera collected at 12-14 weeks pregnancy from 27 controls with uncomplicated pregnancies and 29 cases that later developed preeclampsia. Lipids were extracted and analyzed by direct infusion into a TOF mass spectrometer. MS signals, demonstrating apparent differences were selected, their abundances determined, and statistical differences tested. Statistically significant lipid markers were reevaluated in a second confirmatory study having 43 controls and 37 preeclampsia cases. Multi-marker combinations were developed using those lipid biomarkers confirmed in the second study. The initial study detected 45 potential preeclampsia markers. Of these, 23 markers continued to be statistically significant in the second confirmatory set. Most of these markers, representing several lipid classes, were chemically characterized, typically providing lipid class and potential molecular components using MS(2) Several multi-marker panels with areas under the curve >0.85 and high predictive values were developed. Developed panels of serum lipidomic biomarkers appear to be able to identify most women at risk for preeclampsia in a given pregnancy at 12-14 weeks gestation. |
Databáze: | OpenAIRE |
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