Exogenous application of hydrogen sulfide donor attenuates inflammatory reactions through the L-selectin-involved pathway in the cutaneous reverse passive Arthus reaction
| Autor: | Toshifumi Yamaoka, Kazuhiro Shimizu, Fumihide Ogawa, Eiji Muroi, Toshihide Hara, Koichi Yanaba, Shinichi Sato, Yuichiro Akiyama, Ayumi Yoshizaki |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Neutrophils medicine.drug_class Immunology Gene Expression Endogeny Antigen-Antibody Complex Sulfides Pharmacology Monoclonal antibody Antibodies Nitric oxide Interferon-gamma Mice chemistry.chemical_compound Gene expression Arthus Reaction medicine Animals Immunology and Allergy Hydrogen Sulfide RNA Messenger L-Selectin Skin Inflammation Mice Knockout biology Tumor Necrosis Factor-alpha Arthus reaction Cell Biology medicine.disease Immune complex P-Selectin chemistry biology.protein L-selectin E-Selectin Gene Deletion Selectin Signal Transduction |
| Zdroj: | Journal of Leukocyte Biology. 93:573-584 |
| ISSN: | 1938-3673 0741-5400 |
| DOI: | 10.1189/jlb.0212079 |
| Popis: | H2S donor attenuates inflammatory responses through the L-selectin involved pathway, in the cutaneous reverse passive Arthus reaction. H2S has been highlighted recently as an endogenous, gaseous signaling molecule, especially in inflammations. The deposition of IC induces an acute inflammatory response with tissue injury. To assess the roles of H2S in the IC-induced diseases, the cutaneous, reverse passive Arthus reaction was conducted using NaHS as a H2S donor. Furthermore, we conducted similar experiments using selectin−/− mice to determine the involvement of selectin molecules in the H2S-mediated pathway. Exogenous application of NaHS dramatically attenuated inflammatory reactions in WT mice associated with Arthus reaction. Namely, mRNA expressions of TNF-α, IFN-γ, and neutrophil numbers were reduced significantly in the lesional skins of NaHS-treated WT mice relative to untreated ones. NaHS treatment significantly reduced these three parameters in the lesional skins of E- and P-selectin−/− mice but not in those of L-selectin−/− mice. Quite similar results were obtained in the blocking study using WT mice injected with mAb to E-, P-, and L-selectin. Our results indicated that the exogenous application of NaHS attenuates inflammatory responses in reverse passive Arthus reaction through a L-selectin-involved pathway but not through E- or P-selectin pathways. |
| Databáze: | OpenAIRE |
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