A surgical orthotopic organoid transplantation approach in mice to visualize and study colorectal cancer progression
| Autor: | Saskia J.E. Suijkerbuijk, Evelyne Beerling, Hugo J. Snippert, Harry Begthel, Koen C. Oost, Jacco van Rheenen, Jarno Drost, Arianna Fumagalli |
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| Rok vydání: | 2018 |
| Předmět: |
CRISPR-Cas9 genome editing
0301 basic medicine Mouse Colorectal cancer Biochemistry General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Mice In vivo Mice Inbred NOD Organoid Medicine Animals Humans Stage (cooking) Cancer models Gene Editing Biochemistry Genetics and Molecular Biology(all) business.industry Liver Neoplasms Colonoscopy medicine.disease Xenograft Model Antitumor Assays Epithelium Colon cancer Transplantation Gene Expression Regulation Neoplastic Organoids Disease Models Animal 030104 developmental biology medicine.anatomical_structure Cell Transformation Neoplastic Tumor progression Colonic Neoplasms Cancer research CRISPR-Cas Systems business Colorectal Neoplasms Intravital microscopy Genetics and Molecular Biology(all) |
| Zdroj: | Nature Protocols Nature Protocols, 13(2), 235. Nature Publishing Group |
| ISSN: | 1750-2799 1754-2189 |
| Popis: | Most currently available colorectal cancer (CRC) mouse models are not suitable for studying progression toward the metastatic stage. Recently, establishment of tumor organoid lines, either from murine CRC models or patients, and the possibility of engineering them with genome-editing technologies, have provided a large collection of tumor material faithfully recapitulating phenotypic and genetic heterogeneity of native tumors. To study tumor progression in the natural in vivo environment, we developed an orthotopic approach based on transplantation of CRC organoids into the cecal epithelium. The 20-min procedure is described in detail here and enables growth of transplanted organoids into a single tumor mass within the intestinal tract. Due to long latency, tumor cells are capable of spreading through the blood circulation and forming metastases at distant sites. This method is designed to generate tumors suitable for studying CRC progression, thereby providing the opportunity to visualize tumor cell dynamics in vivo in real time by intravital microscopy. |
| Databáze: | OpenAIRE |
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