The Multispot rapid HIV-1/HIV-2 differentiation assay is comparable with the Western blot and an immunofluorescence assay at confirming HIV infection in a prospective study in three regions of the United States
| Autor: | Mark W. Pandori, Emily Westheimer, Cindy Gay, Nicholas Moss, Jie Fu, Lisa B. Hightow-Weidman, Jason Craw, Laura Hall, Francesca R. Giancotti, Mae Ling Mak, Carmela Madayag, Benjamin Tsoi, Brian Louie, Pragna Patel, S. Michele Owen, Philip J. Peters |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Adolescent Blotting Western Human immunodeficiency virus (HIV) Fluorescent Antibody Technique HIV Infections HIV Antibodies Immunofluorescence medicine.disease_cause 03 medical and health sciences Young Adult 0302 clinical medicine Western blot Antigen Virology medicine Humans Serologic Tests HIV serodiagnosis 030212 general & internal medicine Prospective Studies Prospective cohort study Child Aged Aged 80 and over 0303 health sciences biology medicine.diagnostic_test 030306 microbiology Clinical Laboratory Techniques Diagnostic Tests Routine Screening assay virus diseases HIV Middle Aged Molecular biology United States 3. Good health Infectious Diseases HIV-2 biology.protein HIV-1 Female Antibody True positive rate Algorithms |
| Zdroj: | Journal of Clinical Virology. 58:e92-e96 |
| ISSN: | 1386-6532 |
| DOI: | 10.1016/j.jcv.2013.10.006 |
| Popis: | Background A new HIV diagnostic algorithm has been proposed which replaces the use of the HIV-1 Western blot and HIV-1 immunofluorescence assays (IFA) as the supplemental test with an HIV-1/HIV-2 antibody differentiation assay. Objectives To compare an FDA-approved HIV-1/HIV-2 antibody differentiation test (Multispot) as a confirmatory test with the HIV-1 Western blot and IFA. Study design Participants were screened with an HIV-1/HIV-2 combination Antigen/Antibody (Ag/Ab) screening assay. Specimens with repeatedly reactive results were tested with Multispot and either Western blot or IFA. Specimens with discordant screening and confirmatory results were resolved with HIV-1 RNA testing. Results Individuals (37,876) were screened for HIV infection and 654 (1.7%) had a repeatedly reactive Ag/Ab assay result. On Multispot, 554 (84.7%) were HIV-1 reactive, 0 (0%) were HIV-2 reactive, 1 (0.2%) was reactive for both HIV-1 and HIV-2 (undifferentiated), 9 (1.4%) were HIV-1 indeterminate, and 90 (13.8%) were non-reactive. HIV-1 RNA was detected in 47/90 Multispot non-reactive (52.2%) specimens. Among specimens confirmed to have HIV infection (true positives), Multispot and Western blot detected HIV-1 antibody in a similar proportion of cases (93.7% vs. 94.4% respectively) while Multispot and IFA also detected HIV-1 antibody in a similar proportion of cases (84.5% vs. 83.4% respectively). Conclusions In this study, Multispot confirmed HIV infections at a similar proportion to Western blot and IFA. Multispot, Western blot, and IFA, however, did not confirm all of the reactive Ag/Ab assay results and underscores the importance of HIV NAT testing to resolve discordant screening and confirmatory results. |
| Databáze: | OpenAIRE |
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