Magnaporthe oryzae effectors MoHEG13 and MoHEG16 interfere with host infection and MoHEG13 counteracts cell death caused by Magnaporthe-NLPs in tobacco
| Autor: | Valerie Mogga, Rhoda Delventhal, Denise Weidenbach, Samantha Langer, Philipp M. Bertram, Karsten Andresen, Eckhard Thines, Thomas Kroj, Ulrich Schaffrath |
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| Přispěvatelé: | RWTH Aachen University, Department of Plant Physiology, Rheinisch-Westfälische Technische Hochschule Aachen (RWTH), Kaiserslautern University of Technology, Johannes Gutenberg - Universität Mainz (JGU), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Federal Ministry of Education and Research within the project 'BarleyFortress' |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine effector proteins Plant Science 01 natural sciences magnaporthe oryzae Host Specificity necrosis Fungal Proteins Gene Knockout Techniques 03 medical and health sciences Genes Reporter Tobacco [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Amino Acid Sequence RNA Messenger Plant Diseases Cell Death Virulence fungi Serine Endopeptidases food and beverages barley Hordeum General Medicine Plant Leaves Magnaporthe [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology 030104 developmental biology [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Host-Pathogen Interactions Mutation Mesophyll Cells Sequence Alignment Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant Cell Reports Plant Cell Reports, Springer Verlag, 2016, 35 (5), pp.1169-1185. ⟨10.1007/s00299-016-1943-9⟩ |
| ISSN: | 1432-203X 0721-7714 |
| Popis: | BGPI : équipe 4; International audience; Adapted pathogens are able to modulate cell responses of their hosts most likely due to the activity of secreted effector molecules thereby enabling colonisation by ostensible nonhost pathogens. It is postulated that host and nonhost pathogens of a given plant species differ in their repertoire of secreted effector molecules that are able to suppress plant resistance. We pursued the strategy of identifying novel effectors of Magnaporthe oryzae, the causal agent of blast disease, by comparing the infection process of closely related host vs. nonhost Magnaporthe species on barley (Hordeum vulgare L.). When both types of pathogen simultaneously attacked the same cell, the nonhost isolate became a successful pathogen possibly due to potent effectors secreted by the host isolate. Microarray studies led to a set of M. oryzae Hypothetical Effector Genes (MoHEGs) which were classified as Early- and LateMoHEGs according to the maximal transcript abundance during colonization of barley. Interestingly, orthologs of these MoHEGs from a nonhost pathogen were similarly regulated when investigated in a host situation, suggesting evolutionary conserved functions. Knockout mutants of MoHEG16 from the group of EarlyMoHEGs were less virulent on barley and microscopic studies revealed an attenuated transition from epidermal to mesophyll colonization. MoHEG13, a LateMoHEG, was shown to antagonize cell death induced by M. oryzae Necrosis-and ethylene-inducing-protein-1 (Nep1)-like proteins in Nicotiana benthamiana. MoHEG13 has a virulence function as a knockout mutant showed attenuated disease progression when inoculated on barley. |
| Databáze: | OpenAIRE |
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