Emergence of extended-spectrum beta-lactamases and AmpC-type beta-lactamases in human Salmonella isolated in Spain from 2001 to 2005
| Autor: | Rubén González-Sanz, Manuela de la Fuente, M. Aurora Echeita, Silvia Herrera-León, Margarita Arroyo |
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| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
Adult DNA Bacterial Male Salmonella Adolescent medicine.medical_treatment Salmonella enteritidis Drug resistance Microbial Sensitivity Tests Biology medicine.disease_cause Polymerase Chain Reaction beta-Lactam Resistance beta-Lactamases Microbiology Young Adult Plasmid medicine Pulsed-field gel electrophoresis Escherichia coli Humans Pharmacology (medical) Child Aged Pharmacology Aged 80 and over Infant Sequence Analysis DNA Middle Aged biology.organism_classification Virology Enterobacteriaceae Anti-Bacterial Agents Cephalosporins Electrophoresis Gel Pulsed-Field Infectious Diseases Spain Child Preschool Conjugation Genetic Salmonella Infections Beta-lactamase Female Plasmids |
| Zdroj: | The Journal of antimicrobial chemotherapy. 64(6) |
| ISSN: | 1460-2091 |
| Popis: | Objectives: To study the resistance to third-generation cephalosporins in Salmonella strains isolated from humans in a 5 year period in Spain, and to identify the responsible genes and their dissemination. Methods: Twenty-seven isolates were analysed by PCR and sequencing to identify the genes responsible for the b-lactamase resistance phenotypes. The transferability of the phenotypes was tested by conjugation to Escherichia coli K12J53, plasmid detection with S1-PFGE, hybridization and PCRs of the transconjugants. The genetic relationship was determined by PFGE. Results: We found blaCTX-M-9 and blaCTX-M-10 in Salmonella Virchow PT19. blaCTX-M-14 was detected in Salmonella (IV) 44:z4,z23:-, Salmonella Enteritidis PT6a, Salmonella Typhimurium DT193 and Salmonella Typhimurium DT104B. blaCTX-M-1 was found in Salmonella Litchfield. blaCTX-M-15 and blaCTX-M-32 were found in Salmonella Enteritidis PT1. blaSHV-12 was found in Salmonella Blockley, Salmonella Hadar PT2, Salmonella Enteritidis PT21, Salmonella Enteritidis PT1 and Salmonella Bredeney. blaSHV-2 was found in Salmonella Livingstone. blaCMY-2 was detected in Salmonella Bredeney, Salmonella Newport, Salmonella Enteritidis PT5b and Salmonella Heidelberg. blaDHA-1 was detected for the first time in Spain in Salmonella Newport. One strain of Salmonella Senftenberg harboured two extended-spectrum b-lactamases, blaSHV-12 and blaCTX-M-9. We have found a large variety of b-lactamase families as well as several members of major relevance, such as CTX-M-15, CTX-M-32, CMY-2 and DHA-1. XbaI-PFGE, conjugation assays and S1-PFGE hybridization showed that all these b-lactamases were mediated by plasmids. Conclusions: This study demonstrates the emergence of a public health risk related to resistance to b-lactams in Salmonella. The resistance trends need to be monitored carefully. |
| Databáze: | OpenAIRE |
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