Metabotropic glutamate 1alpha and adenosine A1 receptors assemble into functionally interacting complexes
| Autor: | Rafael Franco, Javier Burgueño, Francisco Ciruela, Wai-Yee Chan, Marisol Escriche, R. A. Jeffrey McIlhinney, Josefa Mallol, Vicent Casadó, Mikhail Soloviev, Carmen Lluis, Enric I. Canela, Ester Angulo |
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| Přispěvatelé: | Universitat de Barcelona |
| Rok vydání: | 2001 |
| Předmět: |
Adenosine
Adenosina Àcid glutàmic Class C GPCR Biology Receptors Metabotropic Glutamate Biochemistry Rhodopsin-like receptors Cell Line Cerebellum Animals Humans Long-term depression Molecular Biology Neural transmission Metabotropic glutamate receptor 6 Receptors Purinergic P1 Cell Biology Receptor Cross-Talk Cell biology Rats Metabotropic receptor Metabotropic glutamate receptor Metabotropic glutamate receptor 1 Neurotransmissió Glutamic acid Metabotropic glutamate receptor 2 Signal Transduction Synaptosomes |
| Zdroj: | Recercat. Dipósit de la Recerca de Catalunya instname Dipòsit Digital de la UB Universidad de Barcelona |
| ISSN: | 0021-9258 |
| Popis: | Recently, evidence has emerged that seven transmembrane G protein-coupled receptors may be present as homo- and heteromers in the plasma membrane. Here we describe a new molecular and functional interaction between two functionally unrelated types of G protein-coupled receptors, namely the metabotropic glutamate type 1alpha (mGlu(1alpha) receptor) and the adenosine A1 receptors in cerebellum, primary cortical neurons, and heterologous transfected cells. Co-immunoprecipitation experiments showed a close and subtype-specific interaction between mGlu(1alpha) and A1 receptors in both rat cerebellar synaptosomes and co-transfected HEK-293 cells. By using transiently transfected HEK-293 cells a synergy between mGlu(1alpha) and A1 receptors in receptor-evoked [Ca(2+)](i) signaling has been shown. In primary cultures of cortical neurons we observed a high degree of co-localization of the two receptors, and excitotoxicity experiments in these cultures also indicate that mGlu(1alpha) and A1 receptors are functionally related. Our results provide a molecular basis for adenosine/glutamate receptors cross-talk and open new perspectives for the development of novel agents to treat neuropsychiatric disorders in which abnormal glutamatergic neurotransmission is involved. |
| Databáze: | OpenAIRE |
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