A redox modulated fluorescence nanoplatform for the detection of alkaline phosphatase activity with fluorescent polydopamine nanoparticles
| Autor: | Jia-Yu He, Rui Yu, Qiang Xiao, Yan-Tong Zhuo, Jing-Xuan Tian, Shuang Wu, Xiang-Juan Kong, Zi-Yan Zhang, Yan-Zhao Fang |
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| Rok vydání: | 2020 |
| Předmět: |
Detection limit
General Chemical Engineering education General Engineering Nanoparticle 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence Redox 0104 chemical sciences Analytical Chemistry Hydrolysis chemistry.chemical_compound chemistry Sodium hydroxide Enzymatic hydrolysis Alkaline phosphatase 0210 nano-technology Nuclear chemistry |
| Zdroj: | Analytical methods : advancing methods and applications. 13(3) |
| ISSN: | 1759-9679 |
| Popis: | Herein, we simply synthesized intrinsic fluorescent polydopamine nanoparticles (PDA NPs) in sodium hydroxide solution (NaOH, pH 11), and constructed a new fluorescence nanoplatform for the detection of alkaline phosphatase (ALP) using PDA NPs as an effective signal reporter. The nanoplatform was constructed by the combination of enzymatic hydrolysis of ALP to the substrate L-ascorbic acid-2-phosphate (AA2P) and the chemical redox reaction between L-ascorbic acid (AA) and mercury ion (Hg2+). The fluorescence of PDA NPs could be effectively quenched by Hg2+ through the coordination effect between Hg2+ and the functional groups on the surface of PDA NPs. However, the quenching effect was greatly inhibited by the addition of AA into the solution. Based on this point, the activity of ALP could be monitored by hydrolysis of the substrate AA2P to AA and the fluorescence output of PDA NPs. The nanoplatform exhibited high sensitivity and desirable selectivity for ALP detection. With a wide linear range of 0 to 18 U L−1, a detection limit of 0.4 U L−1 was achieved using the developed nanosensor. The proposed method could not only be used to screen the inhibitor of ALP but also be used to detect ALP activity in human serum samples successfully. Moreover, the strategy can easily be expanded to determining other kinds of enzymes participating in AA-generation reactions. |
| Databáze: | OpenAIRE |
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