Lewis Antigens and Argyrophilic Nucleolar Organizer Regions Staining for Assessment of Potential Malignancy of Adenomatous Polyps of the Gastrointestinal Tract in Children
| Autor: | Wrzesław Romańczuk, Ryszard Korczowski, Bogdan M. Woźniewicz, Katarzyna Steplewska-Mazur |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Silver Staining Pathology medicine.medical_specialty Nucleolus Immunology Biology Malignancy Familial adenomatous polyposis Lewis Blood Group Antigens Nucleolus Organizer Region Genetics medicine Humans Child Gastrointestinal Neoplasms Gastrointestinal tract Silver Staining Method Antibodies Monoclonal medicine.disease digestive system diseases Staining Adenomatous Polyposis Coli Immunohistochemistry Female Nucleolus organizer region |
| Zdroj: | Hybridoma. 19:269-276 |
| ISSN: | 0272-457X |
| DOI: | 10.1089/02724570050109675 |
| Popis: | Adenomatous polyps (AP) of the gastrointestinal tract in children are very rare. Because of their potential malignancy, they are of great clinical importance. There is little experience in the management of children with AP. The immunohistochemical expression of the Lewis blood group antigens (BGA) (sialosyl-Le(a), Le(a), Leb, Le(x), and Le(y)) and the number of activated nucleoli with the silver staining method for nucleolar organizer regions (AgNORs) were studied in two children with AP. In a girl with isolated AP of the stomach and colon, it was found that antigens Le(b) and s-Le(a) were expressed extensively in the gastric adenoma, and sialosyl-Le(a) throughout the entire length of the rectal adenoma crypts, but in the AgNORs stain the number of nucleoli ranged from two to four, evidencing changes of a benign character. In the case of familial adenomatous polyposis diagnosed in a 9-year-old boy, in some colonic adenomas the number of activated nucleoli was greater than five, and the Le(b) antigen was expressed in superficial epithelial cells in one of the adenomas. Also, extensive expression of antigens Le(y) and s-Le(a) throughout the entire length of the crypt in another polyp removed was observed. We believe that immunohistochemical study of the intensity and extent of the expression of Lewis BGA in the polyp tissue simultaneously with the determination of the number of activated nucleoli by the AgNORs staining method can be helpful in better analysis of cytological risk factors of a malignant transformation. |
| Databáze: | OpenAIRE |
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