A dual-site controlled pH probe revealing the pH of sperm cytoplasm and screening for healthy spermatozoa
Autor: | Xiaoliang Li, Kun Li, Xiao-Qi Yu, Kang-Kang Yu, Sixian Wu, Xiaohui Jiang, Zhiwen Chu, Jianwen Hou, Wenming Xu, Chuan Jiang |
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Rok vydání: | 2021 |
Předmět: |
Male
Cytoplasm endocrine system Intracellular pH Biomedical Engineering Metal Nanoparticles Asthenozoospermia Ph probe Rhodamine chemistry.chemical_compound medicine Humans General Materials Science Fluorescein Fluorescent Dyes Rhodamines urogenital system Optical Imaging General Chemistry General Medicine Hydrogen-Ion Concentration medicine.disease Spermatozoa Sperm Semen Analysis chemistry Colloidal gold Biophysics Gold |
Zdroj: | Journal of Materials Chemistry B. 9:3662-3665 |
ISSN: | 2050-7518 2050-750X |
DOI: | 10.1039/d1tb00108f |
Popis: | A dual-site controlled pH probe, which is composed of gold nanoparticles and modified with rhodamine and fluorescein derivatives, was applied to sensitively monitor intracellular pH changes in sperm. The pH probe revealed the intracellular pH of sperm under different conditions and demonstrated the lower pH in asthenozoospermia patients as compared to healthy individuals. Importantly, the pH probe can help screen for healthy sperm. |
Databáze: | OpenAIRE |
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