Transfer of HBV genomes using low doses of adenovirus vectors leads to persistent infection in immune competent mice
| Autor: | Silke Arzberger, Mathias Heikenwalder, Steffi Graf, Christian Kurts, Percy A. Knolle, Yvonne A. Gäbel, Ulrike Protzer, Li–Rung Huang |
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| Rok vydání: | 2011 |
| Předmět: |
HBsAg
Hepatitis B virus Genetic Vectors Dose-Response Relationship Immunologic medicine.disease_cause Transfection DNA vaccination Viral vector Adenoviridae Hepatitis B Antigens 03 medical and health sciences Mice 0302 clinical medicine Immune system Immunity medicine Animals Seroconversion Hepatitis B Antibodies 030304 developmental biology 0303 health sciences Hepatology business.industry Gastroenterology virus diseases Hepatitis B Virology digestive system diseases Immunity Innate 3. Good health HBcAg Immunology Hepatocytes 030211 gastroenterology & hepatology business Immunocompetence T-Lymphocytes Cytotoxic |
| Zdroj: | Gastroenterology. 142(7) |
| ISSN: | 1528-0012 |
| Popis: | Studies of mechanisms responsible for the persistence of hepatitis B virus (HBV) infection have been hindered by a lack of appropriate animal models. HBV genomes can be delivered to livers of mice using hydrodynamic injection or high doses of an adenoviral vector; these lead to clearance of HBV. We found that infection of immunocompetent mice with low doses of an adenoviral vector resulted in persistent HBV infection; the mice neither underwent seroconversion to production of antibodies against HBV nor developed a strong HBV-specific effector T-cell response. As in patients with chronic HBV infection, DNA vaccination failed to generate T cells that cleared infection. This model of persistent HBV infection could be used to study the pathogenesis of chronic HBV infection and develop new therapeutic strategies. |
| Databáze: | OpenAIRE |
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