Functional Polylactide Blend Films for Controlling Mesenchymal Stem Cell Behaviour

Autor:	Yuliya Nashchekina, Natalya Mikhailova, A. V. Nashchekin, Pavel Nikonov
Rok vydání:	2020
Předmět:	Materials science Polymers and Plastics Scanning electron microscope 02 engineering and technology Polyethylene glycol macromolecular substances 010402 general chemistry 01 natural sciences Article lcsh:QD241-441 chemistry.chemical_compound Polylactic acid lcsh:Organic chemistry Diamine PEG ratio Fourier transform infrared spectroscopy chemistry.chemical_classification mesenchymal stem cells polyethylene glycol diamine technology industry and agriculture General Chemistry Polymer 021001 nanoscience & nanotechnology 0104 chemical sciences blend films Solvent chemistry Chemical engineering polyethylene glycol poly(lactic acid) 0210 nano-technology
Zdroj:	Polymers Polymers, Vol 12, Iss 1969, p 1969 (2020) Volume 12 Issue 9
ISSN:	2073-4360
Popis:	Polymer blending is a suitable physical modification method to create novel properties of different polymers. Blending polylactic acid (PLA) and polyethylene glycol (PEG) produces materials with a wide range of properties. This study was the first to investigate the effect of different isomeric forms of PLA and PEG with terminal amino groups to obtain biocompatible films for human mesenchymal stem cell cultivation. It has been shown by scanning electron microscopy that the surface topology changes to the greatest extent when using films obtained on the basis of poly(d,l-lactide) and PEG with high molecular weights (15,000 g/mol). In order to obtain thin films and rapid evaporation of the solvent, PEG is mixed with PLA and does not form a separate phase and is not further washed out during the incubation in water. The presence of PEG with terminal hydroxyl and amino groups in blend films after incubation in water was proven using Fourier transform infrared (FTIR) spectroscopy. Results of fluorescence microscopy demonstrated that blend films formed on PLA and polyethylene glycol diamine (PEG-NH2) are more suitable for cell spreading and focal contact formation compared to cells cultured on the surface of pure PLA films or films made from PLA and PEG.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab45c0036f7c3e3ff627b8ac013405b4 https://pubmed.ncbi.nlm.nih.gov/32872657 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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