Hyperpolarized xenon-based molecular sensors for label-free detection of analytes
| Autor: | Alexander Pines, Monica Smith, David E. Wemmer, Vikram S. Bajaj, Praveena D. Garimella, Tyler Meldrum, Leah S. Witus, Matthew B. Francis |
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| Rok vydání: | 2013 |
| Předmět: |
Analyte
Xenon Analytical chemistry chemistry.chemical_element Biosensing Techniques Biochemistry Catalysis Chemistry Techniques Analytical Substrate Specificity Rhodamine 6G chemistry.chemical_compound Colloid and Surface Chemistry Limit of Detection Gene Library Inert Staining and Labeling Chemistry Chemical modification General Chemistry Nuclear magnetic resonance spectroscopy Small molecule Magnetic Resonance Imaging Hyperpolarized xenon Colorimetry Peptides |
| Zdroj: | Journal of the American Chemical Society. 136(1) |
| ISSN: | 1520-5126 |
| Popis: | Nuclear magnetic resonance (NMR) can reveal the chemical constituents of a complex mixture without resorting to chemical modification, separation, or other perturbation. Recently, we and others have developed magnetic resonance agents that report on the presence of dilute analytes by proportionately altering the response of a more abundant or easily detected species, a form of amplification. One example of such a sensing medium is xenon gas, which is chemically inert and can be optically hyperpolarized, a process that enhances its NMR signal by up to 5 orders of magnitude. Here, we use a combinatorial synthetic approach to produce xenon magnetic resonance sensors that respond to small molecule analytes. The sensor responds to the ligand by producing a small chemical shift change in the Xe NMR spectrum. We demonstrate this technique for the dye, Rhodamine 6G, for which we have an independent optical assay to verify binding. We thus demonstrate that specific binding of a small molecule can produce a xenon chemical shift change, suggesting a general approach to the production of xenon sensors targeted to small molecule analytes for in vitro assays or molecular imaging in vivo. |
| Databáze: | OpenAIRE |
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